Silybine (SBN), isosilybine (ISBN), silycristine (SCN), silydianine (SDN), and taxifoline (TXF) are the main active flavanoids contained in the dried fruits of Silybum marianum. The concentration of these compounds, excepted TXF, is all together usually expressed as silymarine. In this paper the determination of the content in silymarine was made by high performance liquid chromatography (HPLC), and high performance capillary electrophoresis (HPCE). Two reversed stationary phases, RP-18 and RP-8, were observed comparing the resolutions of all considered flavonoids with each stationary phases. The HPCE was carried out considering the possible improvement in the resolution of SBN, CN, SDN and TXF using,8-cyclodextrines or organic modifier. The qualitative and quantitative data obtained by HPLC and HPCE were compared.
DETERMINATION OF SILYMARINE IN THE EXTRACT FROM THE DRIED SILYBUM MARIANUM FRUITS BY HIGH PERFORMANCE LIQUID CHROMATOGRAPHY AND CAPILLARY ELECTRHOPHORESIS / Quaglia, Maria Giovanna; E., Bossu'; E., Donati; Mazzanti, Gabriela; A., Brandt. - In: JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS. - ISSN 0731-7085. - STAMPA. - 19:3-4(1999), pp. 435-442. [10.1016/S0731-7085(98)00231-3]
DETERMINATION OF SILYMARINE IN THE EXTRACT FROM THE DRIED SILYBUM MARIANUM FRUITS BY HIGH PERFORMANCE LIQUID CHROMATOGRAPHY AND CAPILLARY ELECTRHOPHORESIS
QUAGLIA, Maria Giovanna;MAZZANTI, Gabriela;
1999
Abstract
Silybine (SBN), isosilybine (ISBN), silycristine (SCN), silydianine (SDN), and taxifoline (TXF) are the main active flavanoids contained in the dried fruits of Silybum marianum. The concentration of these compounds, excepted TXF, is all together usually expressed as silymarine. In this paper the determination of the content in silymarine was made by high performance liquid chromatography (HPLC), and high performance capillary electrophoresis (HPCE). Two reversed stationary phases, RP-18 and RP-8, were observed comparing the resolutions of all considered flavonoids with each stationary phases. The HPCE was carried out considering the possible improvement in the resolution of SBN, CN, SDN and TXF using,8-cyclodextrines or organic modifier. The qualitative and quantitative data obtained by HPLC and HPCE were compared.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
