In Parkinson’s disease (PD), alpha-synuclein (a-syn) can be detected in biological fluids including saliva. Although previous studies found reduced a-syn total (a-syntotal) concentration in saliva of PD patients, no studies have previously examined salivary a-syn oligomers (a-synolig) concentrations or assessed the correlation between salivary a-syntotal, a-synolig and clinical features in a large cohort of PD patients. Is well known that a-synolig exerts a crucial neurotoxic effect in PD. We collected salivary samples from 60 PD patients and 40 age- and sex-comparable healthy subjects. PD was diagnosed according to the United Kingdom Brain Bank Criteria. Samples of saliva were analyzed by specific anti-a-syn and anti-oligomeric a-syn ELISA kits. A complete clinical evaluation of each patient was performed using MDS-Unified Parkinson's Disease Rating Scale, Beck Depression Inventory, Montreal Cognitive Assessment and Frontal Assessment Battery. Salivary a-syntotal was lower, whereas a-synolig was higher in PD patients than healthy subjects. The a-synolig/a-syntotal ratio was also higher in patients than in healthy subjects. Salivary a-syntotal concentration negatively correlated with that of a-synolig and correlated with several patients’ clinical features. In PD, decreased salivary concentration of a-syntotal may reflect the reduction of a-syn monomers (a-synmon), as well as the formation of insoluble intracellular inclusions and soluble oligomers. The combined detection of a-syntotal and a-synolig in the saliva might help the early diagnosis of PD

Abnormal salivary total and oligomeric alpha-synuclein in Parkinson's disease / Vivacqua, Giorgio; Latorre, Anna; Suppa, Antonio; Nardi, Michela; Pietracupa, Sara; Mancinelli, Romina; Fabbrini, Giovanni; Colosimo, Carlo; Gaudio, Eugenio; Berardelli, Alfredo. - In: PLOS ONE. - ISSN 1932-6203. - 11:3(2016), pp. 1-12. [10.1371/journal.pone.0151156]

Abnormal salivary total and oligomeric alpha-synuclein in Parkinson's disease

VIVACQUA, GIORGIO;SUPPA, ANTONIO;PIETRACUPA, sara;MANCINELLI, ROMINA;FABBRINI, Giovanni;COLOSIMO, Carlo;GAUDIO, EUGENIO;BERARDELLI, Alfredo
2016

Abstract

In Parkinson’s disease (PD), alpha-synuclein (a-syn) can be detected in biological fluids including saliva. Although previous studies found reduced a-syn total (a-syntotal) concentration in saliva of PD patients, no studies have previously examined salivary a-syn oligomers (a-synolig) concentrations or assessed the correlation between salivary a-syntotal, a-synolig and clinical features in a large cohort of PD patients. Is well known that a-synolig exerts a crucial neurotoxic effect in PD. We collected salivary samples from 60 PD patients and 40 age- and sex-comparable healthy subjects. PD was diagnosed according to the United Kingdom Brain Bank Criteria. Samples of saliva were analyzed by specific anti-a-syn and anti-oligomeric a-syn ELISA kits. A complete clinical evaluation of each patient was performed using MDS-Unified Parkinson's Disease Rating Scale, Beck Depression Inventory, Montreal Cognitive Assessment and Frontal Assessment Battery. Salivary a-syntotal was lower, whereas a-synolig was higher in PD patients than healthy subjects. The a-synolig/a-syntotal ratio was also higher in patients than in healthy subjects. Salivary a-syntotal concentration negatively correlated with that of a-synolig and correlated with several patients’ clinical features. In PD, decreased salivary concentration of a-syntotal may reflect the reduction of a-syn monomers (a-synmon), as well as the formation of insoluble intracellular inclusions and soluble oligomers. The combined detection of a-syntotal and a-synolig in the saliva might help the early diagnosis of PD
adult; aged; aged, 80 and over; biomarkers; cohort studies; early diagnosis; female; humans; male; middle aged; parkinson disease; saliva; solubility; alpha-synuclein; protein aggregates; agricultural and biological sciences (all); biochemistry; genetics and molecular biology (all); medicine (all)
01 Pubblicazione su rivista::01a Articolo in rivista
Abnormal salivary total and oligomeric alpha-synuclein in Parkinson's disease / Vivacqua, Giorgio; Latorre, Anna; Suppa, Antonio; Nardi, Michela; Pietracupa, Sara; Mancinelli, Romina; Fabbrini, Giovanni; Colosimo, Carlo; Gaudio, Eugenio; Berardelli, Alfredo. - In: PLOS ONE. - ISSN 1932-6203. - 11:3(2016), pp. 1-12. [10.1371/journal.pone.0151156]
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11573/884758
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