A gas chromatography method for qualitative and quantitative determination of di-and polyamines from animal tissues was developed. The polyamines were extracted either from tissue homogenate or from standard solution with butanol method, opportunely modified in order to improve both the recoveries and the reproduciblility of the results. Chromatography was performed on a GLC column prepared with Corning glass beads coated with 1% KOH and 4% Carbowax 20 M, with D-amphetamine and benzoamphetamine as internal standard. This column permits a good separation and a nearly complete recovery of polyamines.
A new method for qualitative and quantitative determination of di- and polyamines in animal tissues by gas-liquid chromatography / Beninati, S; Sartori, Claudia; ARGENTO CERÙ, Mp. - In: ANALYTICAL BIOCHEMISTRY. - ISSN 0003-2697. - STAMPA. - 80:(1977), pp. 101-107.
A new method for qualitative and quantitative determination of di- and polyamines in animal tissues by gas-liquid chromatography.
SARTORI, Claudia;
1977
Abstract
A gas chromatography method for qualitative and quantitative determination of di-and polyamines from animal tissues was developed. The polyamines were extracted either from tissue homogenate or from standard solution with butanol method, opportunely modified in order to improve both the recoveries and the reproduciblility of the results. Chromatography was performed on a GLC column prepared with Corning glass beads coated with 1% KOH and 4% Carbowax 20 M, with D-amphetamine and benzoamphetamine as internal standard. This column permits a good separation and a nearly complete recovery of polyamines.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
