Macrophages’ phenotypic and functional diversity depends on differentiating programs being Great interest was addressed to identify the signal transduction pathways acting in macrophage polarization, including the phosphoinositide (PI) system and related phospholipase C (PLC) family of enzymes. PLCs are strictly tissue specific and the expression panel, as well as the subcellular localization differs in quiescent cells compared to the pathological counterpart. We analyzed the expression of PLC enzymes in unpolarized (M0), M1 and M2 macrophages to list the isoforms expressed in the polarized macrophages and their subcellular localization. Our results confirmed that macrophages express a wide number of PLC isoforms. All PLC enzymes were detected within both M1 and M2 cells, but not in M0 cells. M0, as well as M1 and M2 cells own a specific panel of expression, different for both genes’ mRNA expression and intracellular localization of PLC enzymes. PLC enzymes might play a complex role in macrophages during inflammation and probably also during polarization.
Expression of Phosphoinositide-specific Phospholipase C enzymes in polarized macrophages / DI RAIMO, Tania; Leopizzi, Martina; Businaro, Rita; Rita, ; DELLA ROCCA, Carlo; Carlo, ; Laskin, Debra; LO VASCO, VINCENZA RITA; Vincenza, Rita. - STAMPA. - (2016). (Intervento presentato al convegno Cell Symposia. 100 Years Phagocytes tenutosi a Giardini Naxos nel 2016, Septembre, 19-22th).
Expression of Phosphoinositide-specific Phospholipase C enzymes in polarized macrophages
DI RAIMO, TANIA;LEOPIZZI, MARTINA;BUSINARO, Rita;DELLA ROCCA, Carlo;LO VASCO, VINCENZA RITA;
2016
Abstract
Macrophages’ phenotypic and functional diversity depends on differentiating programs being Great interest was addressed to identify the signal transduction pathways acting in macrophage polarization, including the phosphoinositide (PI) system and related phospholipase C (PLC) family of enzymes. PLCs are strictly tissue specific and the expression panel, as well as the subcellular localization differs in quiescent cells compared to the pathological counterpart. We analyzed the expression of PLC enzymes in unpolarized (M0), M1 and M2 macrophages to list the isoforms expressed in the polarized macrophages and their subcellular localization. Our results confirmed that macrophages express a wide number of PLC isoforms. All PLC enzymes were detected within both M1 and M2 cells, but not in M0 cells. M0, as well as M1 and M2 cells own a specific panel of expression, different for both genes’ mRNA expression and intracellular localization of PLC enzymes. PLC enzymes might play a complex role in macrophages during inflammation and probably also during polarization.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
