A subcellular fractionation procedure for frog liver is reported and validated by the distribution pattern of several marker enzymes, also in comparison with rat liver. 2. The subcellular distribution of tyrosine aminotransferase was investigated in frog liver as compared to rat liver: a different distribution of the enzyme was observed, being the activity mostly recovered in mitochondrial and cytosolic compartments. 3. Results indicate that mitochondrial tyrosine aminotransferase of both frog and rat liver is a matrix enzyme, even if differences are observed concerning its release from the organelles upon detergent treatment.
Tyrosine aminotransferase activity of frog (Rana esculenta) liver--II. Comparative aspects of intracellular distribution / Scapin, S; Autuori, F; Baldini, P; Incerpi, S; Luly, P; Sartori, Claudia. - In: COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY. A. COMPARATIVE PHYSIOLOGY. - ISSN 0300-9629. - STAMPA. - 73:(1982), pp. 779-783.
Tyrosine aminotransferase activity of frog (Rana esculenta) liver--II. Comparative aspects of intracellular distribution.
SARTORI, Claudia
1982
Abstract
A subcellular fractionation procedure for frog liver is reported and validated by the distribution pattern of several marker enzymes, also in comparison with rat liver. 2. The subcellular distribution of tyrosine aminotransferase was investigated in frog liver as compared to rat liver: a different distribution of the enzyme was observed, being the activity mostly recovered in mitochondrial and cytosolic compartments. 3. Results indicate that mitochondrial tyrosine aminotransferase of both frog and rat liver is a matrix enzyme, even if differences are observed concerning its release from the organelles upon detergent treatment.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
