Cytofluorimetric and reverse-transcription polymerase chain reaction (RT-PCR) analysis showed that adriamycin-resistant (ADR(R)), but not sensitive (WT), MCF-7 human mammary carcinoma cell lines express alpha 4 beta 1 and alpha 5 beta 1 integrins. ADR(R) cells adhere to fibronectin (FN), and only alpha 5 beta 1 is involved in cell adhesion to this glycoprotein, while alpha 4 beta 1 mediates cell binding to the cellular counter-receptor VCAM-1. proliferation assays showed that FN, but not VCAM-1, delivers a mitogenic signal to quiescent ADR(R) MCF-7 cells. The activating signal is mediated by alpha 5 beta 1, since cell proliferation is inhibited in the presence of RGD peptide or specific antibody. Cell cycle analysis demonstrated that cell/FN interaction induces the re-entry of ADR(R) MCF-7 into 5 phase, and prevents them from undergoing serum deprivation-induced apoptosis. Our data suggest that the presence of alpha 5 beta 1 on the resistant cells enables them to draw advantage from FN for both cell growth and survival. (C) 1997 Wiley-Liss, Inc.
Functional role of alpha 4 beta 1 and alpha 5 beta 1 integrin fibronectin receptors expressed on adriamycin-resistant MCF-7 human mammary carcinoma cells / A., Nista; C., Leonetti; Bernardini, Giovanni; M., Mattioni; Santoni, Angela. - In: INTERNATIONAL JOURNAL OF CANCER. - ISSN 0020-7136. - STAMPA. - 72:(1997), pp. 133-141. [10.1002/(sici)1097-0215(19970703)72:1<133::aid-ijc19>3.0.co;2-k]
Functional role of alpha 4 beta 1 and alpha 5 beta 1 integrin fibronectin receptors expressed on adriamycin-resistant MCF-7 human mammary carcinoma cells
BERNARDINI, Giovanni;SANTONI, Angela
1997
Abstract
Cytofluorimetric and reverse-transcription polymerase chain reaction (RT-PCR) analysis showed that adriamycin-resistant (ADR(R)), but not sensitive (WT), MCF-7 human mammary carcinoma cell lines express alpha 4 beta 1 and alpha 5 beta 1 integrins. ADR(R) cells adhere to fibronectin (FN), and only alpha 5 beta 1 is involved in cell adhesion to this glycoprotein, while alpha 4 beta 1 mediates cell binding to the cellular counter-receptor VCAM-1. proliferation assays showed that FN, but not VCAM-1, delivers a mitogenic signal to quiescent ADR(R) MCF-7 cells. The activating signal is mediated by alpha 5 beta 1, since cell proliferation is inhibited in the presence of RGD peptide or specific antibody. Cell cycle analysis demonstrated that cell/FN interaction induces the re-entry of ADR(R) MCF-7 into 5 phase, and prevents them from undergoing serum deprivation-induced apoptosis. Our data suggest that the presence of alpha 5 beta 1 on the resistant cells enables them to draw advantage from FN for both cell growth and survival. (C) 1997 Wiley-Liss, Inc.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.