Plant protein inhibitors counteract the activity of cell wall-degrading enzymes (CWDEs) secreted by pathogens to breach the plant cell-wall barrier. Transgenic plants expressing a single protein inhibitor restrict pathogen infections. However, since pathogens secrete a number of CWDEs at the onset of infection, we combined more inhibitors in a single wheat genotype to reinforce further the cell-wall barrier. We combined polygalacturonase (PG) inhibiting protein (PGIP) and pectin methyl esterase inhibitor (PMEI), both controlling the activity of PG, one of the first CWDEs secreted during infection. We also pyramided PGIP and TAXI-III, a xylanase inhibitor that controls the activity of xylanases, key factors for the degradation of xylan, a main component of cereal cell wall. We demonstrated that the pyramiding of PGIP and PMEI did not contribute to any further improvement of disease resistance. However, the presence of both pectinase inhibitors ensured a broader spectrum of disease resistance. Conversely, the PGIP and TAXI-III combination contributed to further improvement of Fusarium head blight (FHB) resistance, probably because these inhibitors target the activity of different types of CWDEs, i.e., PGs and xylanases. Worth mentioning, the reduction of FHB symptoms is accompanied by a reduction of deoxynivalenol accumulation with a foreseen great benefit to human and animal health.

Pyramiding PvPGIP2 and TAXI-III but not PvPGIP2 and PMEI enhances resistance against Fusarium graminearum / Tundo, S; Kalunke, R; Janni, M; Volpi, C; Lionetti, Vincenzo; Bellincampi, Daniela; Favaron, F; D'Ovidio, R.. - In: MOLECULAR PLANT-MICROBE INTERACTIONS. - ISSN 0894-0282. - STAMPA. - 29:8(2016), pp. 629-639. [10.1094/MPMI-05-16-0089-R]

Pyramiding PvPGIP2 and TAXI-III but not PvPGIP2 and PMEI enhances resistance against Fusarium graminearum

LIONETTI, VINCENZO;BELLINCAMPI, Daniela;
2016

Abstract

Plant protein inhibitors counteract the activity of cell wall-degrading enzymes (CWDEs) secreted by pathogens to breach the plant cell-wall barrier. Transgenic plants expressing a single protein inhibitor restrict pathogen infections. However, since pathogens secrete a number of CWDEs at the onset of infection, we combined more inhibitors in a single wheat genotype to reinforce further the cell-wall barrier. We combined polygalacturonase (PG) inhibiting protein (PGIP) and pectin methyl esterase inhibitor (PMEI), both controlling the activity of PG, one of the first CWDEs secreted during infection. We also pyramided PGIP and TAXI-III, a xylanase inhibitor that controls the activity of xylanases, key factors for the degradation of xylan, a main component of cereal cell wall. We demonstrated that the pyramiding of PGIP and PMEI did not contribute to any further improvement of disease resistance. However, the presence of both pectinase inhibitors ensured a broader spectrum of disease resistance. Conversely, the PGIP and TAXI-III combination contributed to further improvement of Fusarium head blight (FHB) resistance, probably because these inhibitors target the activity of different types of CWDEs, i.e., PGs and xylanases. Worth mentioning, the reduction of FHB symptoms is accompanied by a reduction of deoxynivalenol accumulation with a foreseen great benefit to human and animal health.
2016
Plant cell wall; wheat; Fusarium
01 Pubblicazione su rivista::01a Articolo in rivista
Pyramiding PvPGIP2 and TAXI-III but not PvPGIP2 and PMEI enhances resistance against Fusarium graminearum / Tundo, S; Kalunke, R; Janni, M; Volpi, C; Lionetti, Vincenzo; Bellincampi, Daniela; Favaron, F; D'Ovidio, R.. - In: MOLECULAR PLANT-MICROBE INTERACTIONS. - ISSN 0894-0282. - STAMPA. - 29:8(2016), pp. 629-639. [10.1094/MPMI-05-16-0089-R]
File allegati a questo prodotto
File Dimensione Formato  
Tundo_Pyramiding_2016.pdf

accesso aperto

Note: Articolo principale
Tipologia: Versione editoriale (versione pubblicata con il layout dell'editore)
Licenza: Tutti i diritti riservati (All rights reserved)
Dimensione 797.8 kB
Formato Adobe PDF
797.8 kB Adobe PDF

I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.

Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11573/878330
Citazioni
  • ???jsp.display-item.citation.pmc??? 10
  • Scopus 24
  • ???jsp.display-item.citation.isi??? 23
social impact