In this work we studied the local anaesthetic activity of beta-caryophyllene, one of the main components of clove oil obtained from the dried flower-buds of Syzygium aromaticum (Myrtaceae family). We compared its activity to a chemically related compound, caryophyllene oxide. Anaesthetic activity was evaluated in vivo in the rabbit conjunctival reflex test and in vitro in a rat phrenic nerve-hemidiaphragm preparation. beta-Caryophyllene (10(-4)-1 mug/ml), but not caryophyllene oxide, was able to reduce drastically, in a dose-dependent manner, the electrically evoked contractions of the rat phrenic hemidiaphragm. In the rabbit, conjunctival reflex test treatment with a solution of beta-caryophyllene (10-1000 mug/ml) allowed a dose-dependent increase in the number of stimuli necessary to provoke the reflex. As in the in vitro results, caryophyllene oxide was ineffective also in the in vivo test. In conclusion, these data evidence the local anaesthetic activity of beta-caryophyllene, which appears to be strictly dependent on its chemical structure. (C) 2001 Editions scientifiques et medicales Elsevier SAS.
Local anaesthetic activity of beta-caryophyllene / C., Ghelardini; N., Galeotti; L., Di Cesare Mannelli; Mazzanti, Gabriela; A., Bartolini. - In: IL FARMACO. - ISSN 0014-827X. - STAMPA. - 56:(2001), pp. 387-389. ( Workshop of the Italian-Society-of-Pharmacognosy (SIPHAR) ASSISI, ITALY SEP 19, 2000) [10.1016/s0014-827x(01)01092-8].
Local anaesthetic activity of beta-caryophyllene
MAZZANTI, Gabriela;
2001
Abstract
In this work we studied the local anaesthetic activity of beta-caryophyllene, one of the main components of clove oil obtained from the dried flower-buds of Syzygium aromaticum (Myrtaceae family). We compared its activity to a chemically related compound, caryophyllene oxide. Anaesthetic activity was evaluated in vivo in the rabbit conjunctival reflex test and in vitro in a rat phrenic nerve-hemidiaphragm preparation. beta-Caryophyllene (10(-4)-1 mug/ml), but not caryophyllene oxide, was able to reduce drastically, in a dose-dependent manner, the electrically evoked contractions of the rat phrenic hemidiaphragm. In the rabbit, conjunctival reflex test treatment with a solution of beta-caryophyllene (10-1000 mug/ml) allowed a dose-dependent increase in the number of stimuli necessary to provoke the reflex. As in the in vitro results, caryophyllene oxide was ineffective also in the in vivo test. In conclusion, these data evidence the local anaesthetic activity of beta-caryophyllene, which appears to be strictly dependent on its chemical structure. (C) 2001 Editions scientifiques et medicales Elsevier SAS.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
