Transcriptome analysis allowed the identification of new long noncoding RNAs differentially expressed during murine myoblast differentiation. These transcripts were classified on the basis of their expression under proliferating versus differentiated conditions, muscle-restricted activation, and subcellular localization. Several species displayed preferential expression in dystrophic (mdx) versus wild-type muscles, indicating their possible link with regenerative processes. One of the identified transcripts, lnc-31, even if originating from the same nuclear precursor of miR-31, is produced by a pathway mutually exclusive. We show that lnc-31 and its human homologue hsa-lnc-31 are expressed in proliferating myoblasts, where they counteract differentiation. In line with this, both species are more abundant in mdx muscles and in human Duchenne muscular dystrophy (DMD) myoblasts, than in their normal counterparts. Altogether, these data suggest a crucial role for lnc-31 in controlling the differentiation commitment of precursor myoblasts and indicate that its function is maintained in evolution despite the poor sequence conservation with the human counterpart.

Novel long noncoding RNAs (lncRNAs) in Myogenesis: A miR-31 overlapping lncRNA transcript controls myoblast differentiation / Ballarino, Monica; Cazzella, Valentina; D'Andrea, Daniel; Grassi, Luigi; Bisceglie, Lavinia; Cipriano, Andrea; Santini, Tiziana; Pinnaro', Chiara; Morlando, Mariangela; Tramontano, Anna; Bozzoni, Irene. - In: MOLECULAR AND CELLULAR BIOLOGY. - ISSN 0270-7306. - ELETTRONICO. - 35:4(2015), pp. 728-736. [10.1128/MCB.01394-14]

Novel long noncoding RNAs (lncRNAs) in Myogenesis: A miR-31 overlapping lncRNA transcript controls myoblast differentiation

BALLARINO, MONICA;CAZZELLA, VALENTINA;D'ANDREA, DANIEL;GRASSI, LUIGI;CIPRIANO, ANDREA;SANTINI, Tiziana;PINNARO', CHIARA;MORLANDO, MARIANGELA;TRAMONTANO, ANNA;BOZZONI, Irene
2015

Abstract

Transcriptome analysis allowed the identification of new long noncoding RNAs differentially expressed during murine myoblast differentiation. These transcripts were classified on the basis of their expression under proliferating versus differentiated conditions, muscle-restricted activation, and subcellular localization. Several species displayed preferential expression in dystrophic (mdx) versus wild-type muscles, indicating their possible link with regenerative processes. One of the identified transcripts, lnc-31, even if originating from the same nuclear precursor of miR-31, is produced by a pathway mutually exclusive. We show that lnc-31 and its human homologue hsa-lnc-31 are expressed in proliferating myoblasts, where they counteract differentiation. In line with this, both species are more abundant in mdx muscles and in human Duchenne muscular dystrophy (DMD) myoblasts, than in their normal counterparts. Altogether, these data suggest a crucial role for lnc-31 in controlling the differentiation commitment of precursor myoblasts and indicate that its function is maintained in evolution despite the poor sequence conservation with the human counterpart.
2015
Animals; Biological Evolution; Cell Differentiation; Cell Proliferation; Gene Expression Profiling; Gene Expression Regulation; Humans; Mice; Mice, Transgenic; MicroRNAs; Muscle Development; Muscle, Smooth; Muscle, Striated; Muscular Dystrophy, Duchenne; Myoblasts; RNA Precursors; RNA, Long Noncoding; Transcriptome; Molecular Biology; Cell Biology
01 Pubblicazione su rivista::01a Articolo in rivista
Novel long noncoding RNAs (lncRNAs) in Myogenesis: A miR-31 overlapping lncRNA transcript controls myoblast differentiation / Ballarino, Monica; Cazzella, Valentina; D'Andrea, Daniel; Grassi, Luigi; Bisceglie, Lavinia; Cipriano, Andrea; Santini, Tiziana; Pinnaro', Chiara; Morlando, Mariangela; Tramontano, Anna; Bozzoni, Irene. - In: MOLECULAR AND CELLULAR BIOLOGY. - ISSN 0270-7306. - ELETTRONICO. - 35:4(2015), pp. 728-736. [10.1128/MCB.01394-14]
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11573/871673
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