In this study, we report the development of an SPR (Surface Plasmon Resonance) immunosensor for the detection of ampicillin, operating under flow conditions. SPR sensors based on both direct (with the immobilization of the antibody) and competitive (with the immobilization of the antigen) methods did not allow the detection of ampicillin. Therefore, a sandwich-based sensor was developed which showed a good linear response towards ampicillin between 10-3 and 10-1 M, a measurement time of ≤20 min and a high selectivity both towardsβ-lactam antibiotics and antibiotics of different classes. © 2016 by the author.
A flow SPR immunosensor based on a sandwich direct method / Tomassetti, Mauro; Conta, Giorgia; Campanella, Luigi; Favero, Gabriele; Sanzo', Gabriella; Mazzei, Franco; Antiochia, Riccarda. - In: BIOSENSORS. - ISSN 2079-6374. - STAMPA. - 6:2(2016). [10.3390/bios6020022]
A flow SPR immunosensor based on a sandwich direct method
TOMASSETTI, Mauro;CAMPANELLA, Luigi;FAVERO, Gabriele;SANZO', GABRIELLA;MAZZEI, Franco;ANTIOCHIA, RICCARDA
2016
Abstract
In this study, we report the development of an SPR (Surface Plasmon Resonance) immunosensor for the detection of ampicillin, operating under flow conditions. SPR sensors based on both direct (with the immobilization of the antibody) and competitive (with the immobilization of the antigen) methods did not allow the detection of ampicillin. Therefore, a sandwich-based sensor was developed which showed a good linear response towards ampicillin between 10-3 and 10-1 M, a measurement time of ≤20 min and a high selectivity both towardsβ-lactam antibiotics and antibiotics of different classes. © 2016 by the author.| File | Dimensione | Formato | |
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