The evaluation of oral fluids (OFs) levels is useful in proving drug consumption, particularly for monitoring abuse in workplaces and for the driving under the influence of drugs (DUIDs) programs. OF is a complex matrix and a small amount of sample is available, especially after cannabis smoking. This paper reports a method for the determination of cannabinoids and metabolites in OF: THC, 11-hydroxy-THC (OH-THC) and THC-COOH. Cannabidiol (CBD) and cannabinol (CBN) were also detected by LC-MS/MS. The OF pre-treatment was based on micro-extraction by packed sorbent (MEPS), a recently developed solid phase extraction technique which operates with small sample volumes: only 125μL of sample was required, allowing the collection by simple expectoration. Analytes elution was achieved using 2×25μL of 50mM NH4OH in methanol. A rapid and effective clean-up has been obtained with satisfactory recovery values and a negligible matrix effect. The LOQs ranged between 0.020ngmL-1 for THC-COOH and 0.40ngmL-1 for OH-THC. The chromatographic conditions obtained with a fused-core column allowed a good separation of the analytes in 6min only. The whole procedure has been validated according to SOFT/AAFS guidelines. © 2013 Elsevier B.V.
Micro extraction by packed sorbent coupled to liquid chromatography tandem mass spectrometry for the rapid and sensitive determination of cannabinoids in oral fluids / Sergi, M.; Montesano, Camilla; Odoardi, S.; Mainero Rocca, L.; Fabrizi, G.; Compagnone, D.; Curini, Roberta. - In: JOURNAL OF CHROMATOGRAPHY A. - ISSN 0021-9673. - STAMPA. - 1301:(2013), pp. 139-146. [10.1016/j.chroma.2013.05.072]
Micro extraction by packed sorbent coupled to liquid chromatography tandem mass spectrometry for the rapid and sensitive determination of cannabinoids in oral fluids
Sergi, M.;MONTESANO, CAMILLA;CURINI, Roberta
2013
Abstract
The evaluation of oral fluids (OFs) levels is useful in proving drug consumption, particularly for monitoring abuse in workplaces and for the driving under the influence of drugs (DUIDs) programs. OF is a complex matrix and a small amount of sample is available, especially after cannabis smoking. This paper reports a method for the determination of cannabinoids and metabolites in OF: THC, 11-hydroxy-THC (OH-THC) and THC-COOH. Cannabidiol (CBD) and cannabinol (CBN) were also detected by LC-MS/MS. The OF pre-treatment was based on micro-extraction by packed sorbent (MEPS), a recently developed solid phase extraction technique which operates with small sample volumes: only 125μL of sample was required, allowing the collection by simple expectoration. Analytes elution was achieved using 2×25μL of 50mM NH4OH in methanol. A rapid and effective clean-up has been obtained with satisfactory recovery values and a negligible matrix effect. The LOQs ranged between 0.020ngmL-1 for THC-COOH and 0.40ngmL-1 for OH-THC. The chromatographic conditions obtained with a fused-core column allowed a good separation of the analytes in 6min only. The whole procedure has been validated according to SOFT/AAFS guidelines. © 2013 Elsevier B.V.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
