Oral fluid (OF) has become a valuable biologic specimen for toxicological analysis, especially in driving under the influence of drugs (DUID) investigations, because of easy and non-invasive collection procedures. In OF testing, being the sample volume is limited, multi-analyte procedures are particularly advantageous since they save time and resources. In this work, a procedure for the simultaneous analysis of 20 illicit drugs, belonging to the classes of cocaine, amphetamines, natural and synthetic opioids and hallucinogens, is presented. The sample preparation is based on microextraction by packed sorbent (MEPS), a novel technique which is based on the miniaturization of solid-phase extraction (SPE). The presented method, which includes all the most diffused illicit drugs and their metabolites, has been fully validated according to the Scientific Working Group for Forensic Toxicology (SWGTOX) guidelines. LLOQs ranged from 0.5 to 30 ng mL(-1) (diacetylmorphine); the presented method allows the detection of all the selected drugs quite below the cutoff values recommended by Substance Abuse and Mental Health Services Administration (SAMHSA) for abuse identification

Determination of illicit drugs and metabolites in oral fluid by microextraction on packed sorbent coupled with LC-MS/MS / Montesano, Camilla; Simeoni, Maria Chiara; Curini, Roberta; Sergi, Manuel; Lo Sterzo, Claudio; Compagnone, Dario. - In: ANALYTICAL AND BIOANALYTICAL CHEMISTRY. - ISSN 1618-2650. - 407:13(2015), pp. 3647-3658. [10.1007/s00216-015-8583-8]

Determination of illicit drugs and metabolites in oral fluid by microextraction on packed sorbent coupled with LC-MS/MS

MONTESANO, CAMILLA;CURINI, Roberta;Sergi, Manuel
;
2015

Abstract

Oral fluid (OF) has become a valuable biologic specimen for toxicological analysis, especially in driving under the influence of drugs (DUID) investigations, because of easy and non-invasive collection procedures. In OF testing, being the sample volume is limited, multi-analyte procedures are particularly advantageous since they save time and resources. In this work, a procedure for the simultaneous analysis of 20 illicit drugs, belonging to the classes of cocaine, amphetamines, natural and synthetic opioids and hallucinogens, is presented. The sample preparation is based on microextraction by packed sorbent (MEPS), a novel technique which is based on the miniaturization of solid-phase extraction (SPE). The presented method, which includes all the most diffused illicit drugs and their metabolites, has been fully validated according to the Scientific Working Group for Forensic Toxicology (SWGTOX) guidelines. LLOQs ranged from 0.5 to 30 ng mL(-1) (diacetylmorphine); the presented method allows the detection of all the selected drugs quite below the cutoff values recommended by Substance Abuse and Mental Health Services Administration (SAMHSA) for abuse identification
2015
high pressure liquid chromatography; saliva; micro extraction on packed sorbent
01 Pubblicazione su rivista::01a Articolo in rivista
Determination of illicit drugs and metabolites in oral fluid by microextraction on packed sorbent coupled with LC-MS/MS / Montesano, Camilla; Simeoni, Maria Chiara; Curini, Roberta; Sergi, Manuel; Lo Sterzo, Claudio; Compagnone, Dario. - In: ANALYTICAL AND BIOANALYTICAL CHEMISTRY. - ISSN 1618-2650. - 407:13(2015), pp. 3647-3658. [10.1007/s00216-015-8583-8]
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11573/871264
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