The aim of this study was to investigate the relevance and the robustness of active and passive approaches used in freshwater biomonitoring with the ecologically relevant gammarid amphipod Echinogammarus veneris. To assess the contaminant bioavailability in two rivers of Latium (Central Italy), we measured the genotoxic potential in haemocytes by comet assay and metal bioaccumulation in tissues by analytical methods. We adopted an active strategy of exposure in situ and a passive method of sampling in situ. In the first case, the gammarids were exposed in cages in several sampling sites selected along two rivers, while in the sampling in situ, individuals were collected directly in the same sampling sites and then analyzed. The results indicate that the comet assay carried out on haemocytes from caged individuals proved to be a sensitive tool for freshwater genotoxicity monitoring. However, the sampling in situ is more appropriate for a realistic understanding of the presence of trace metal in E. veneris.

Comparison between active and passive biomonitoring strategies for the assessment of genotoxicity and metal bioaccumulation in Echinogammarus veneris (Crustacea: Amphipoda) / Ronci, L.; Meccoli, L.; Iannilli, V.; Menegoni, Patrizia; De Matthaeis, E.; Setini, A.. - In: THE ITALIAN JOURNAL OF ZOOLOGY. - ISSN 1125-0003. - ELETTRONICO. - 83:2(2016), pp. 1-11. [10.1080/11250003.2016.1169321]

Comparison between active and passive biomonitoring strategies for the assessment of genotoxicity and metal bioaccumulation in Echinogammarus veneris (Crustacea: Amphipoda)

L. Ronci
Writing – Original Draft Preparation
;
MENEGONI, Patrizia;E. De Matthaeis
Supervision
;
A. Setini
Methodology
2016

Abstract

The aim of this study was to investigate the relevance and the robustness of active and passive approaches used in freshwater biomonitoring with the ecologically relevant gammarid amphipod Echinogammarus veneris. To assess the contaminant bioavailability in two rivers of Latium (Central Italy), we measured the genotoxic potential in haemocytes by comet assay and metal bioaccumulation in tissues by analytical methods. We adopted an active strategy of exposure in situ and a passive method of sampling in situ. In the first case, the gammarids were exposed in cages in several sampling sites selected along two rivers, while in the sampling in situ, individuals were collected directly in the same sampling sites and then analyzed. The results indicate that the comet assay carried out on haemocytes from caged individuals proved to be a sensitive tool for freshwater genotoxicity monitoring. However, the sampling in situ is more appropriate for a realistic understanding of the presence of trace metal in E. veneris.
2016
gammaridae; comet assay; biomarker; in situ assessment; fresh water quality
01 Pubblicazione su rivista::01a Articolo in rivista
Comparison between active and passive biomonitoring strategies for the assessment of genotoxicity and metal bioaccumulation in Echinogammarus veneris (Crustacea: Amphipoda) / Ronci, L.; Meccoli, L.; Iannilli, V.; Menegoni, Patrizia; De Matthaeis, E.; Setini, A.. - In: THE ITALIAN JOURNAL OF ZOOLOGY. - ISSN 1125-0003. - ELETTRONICO. - 83:2(2016), pp. 1-11. [10.1080/11250003.2016.1169321]
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11573/871010
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