A statistical mixture design approach was used to investigate the effects of cell wall degrading enzymes on the recovery of lipids from Nannochloropsis sp. A preliminary screening of potentially suitable enzyme preparations, including lysozyme, cellulase and different types of hemicellulases, was carried out. The most effective preparations were then taken as basic components for the formulation of enzyme mixtures. Optimized ternary mixtures consisting of cellulase and two hemicellulases were obtained which allowed the recovery of up to 37.2 g of lipids per 100 g of dry biomass. SEM and TEM images of the enzymatically treated microalga revealed extensive cell damage, with degradation of the cell wall and release of intracellular material. Overall, the results obtained demonstrate that the mixture design method can be used to prepare cell wall degrading enzyme cocktails that can significantly improve the recovery of lipids or other valuable components from microalgae

Enhanced lipid recovery from nannochloropsis microalgae by treatment with optimized cell wall degrading enzyme mixtures / Zuorro, Antonio; Miglietta, Selenia; Familiari, Giuseppe; Lavecchia, Roberto. - In: BIORESOURCE TECHNOLOGY. - ISSN 0960-8524. - STAMPA. - 212:(2016), pp. 35-41. [10.1016/j.biortech.2016.04.025]

Enhanced lipid recovery from nannochloropsis microalgae by treatment with optimized cell wall degrading enzyme mixtures

ZUORRO, ANTONIO;MIGLIETTA, SELENIA;FAMILIARI, Giuseppe;LAVECCHIA, Roberto
2016

Abstract

A statistical mixture design approach was used to investigate the effects of cell wall degrading enzymes on the recovery of lipids from Nannochloropsis sp. A preliminary screening of potentially suitable enzyme preparations, including lysozyme, cellulase and different types of hemicellulases, was carried out. The most effective preparations were then taken as basic components for the formulation of enzyme mixtures. Optimized ternary mixtures consisting of cellulase and two hemicellulases were obtained which allowed the recovery of up to 37.2 g of lipids per 100 g of dry biomass. SEM and TEM images of the enzymatically treated microalga revealed extensive cell damage, with degradation of the cell wall and release of intracellular material. Overall, the results obtained demonstrate that the mixture design method can be used to prepare cell wall degrading enzyme cocktails that can significantly improve the recovery of lipids or other valuable components from microalgae
2016
cell wall degrading enzymes; lipid recovery; mixture design; nannochloropsis; optimization; bioengineering; environmental engineering; waste management and disposal
01 Pubblicazione su rivista::01a Articolo in rivista
Enhanced lipid recovery from nannochloropsis microalgae by treatment with optimized cell wall degrading enzyme mixtures / Zuorro, Antonio; Miglietta, Selenia; Familiari, Giuseppe; Lavecchia, Roberto. - In: BIORESOURCE TECHNOLOGY. - ISSN 0960-8524. - STAMPA. - 212:(2016), pp. 35-41. [10.1016/j.biortech.2016.04.025]
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11573/866045
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