Telomeric Repeat Amplification Protocol (TRAP) is a sensitive procedure to measure telomerase activity in small samples of cell or tissue extracts. Due to the strict correlation between high levels of telomerase activity and neoplastic transformation, TRAP assay could provide an important diagnostic marker of malignancy. Although the original TRAP assay is very Sensitive and some improvements have been described, occasional artifacts still persist in the modified procedures. Here we describe how changes in the sequence of the primer used dor the amplification step enhance the reproducibility and sensitivity in the TRAP assay.
Increased sensitivity and reproducibility of TRAP assay by avoiding direct primers interaction / M. L., Falchetti; A., Levi; P., Molinari; Verna, Roberto; E., D'Ambrosio. - In: NUCLEIC ACIDS RESEARCH. - ISSN 0305-1048. - STAMPA. - 26:3(1998), pp. 862-863. [10.1093/nar/26.3.862]
Increased sensitivity and reproducibility of TRAP assay by avoiding direct primers interaction
VERNA, Roberto;
1998
Abstract
Telomeric Repeat Amplification Protocol (TRAP) is a sensitive procedure to measure telomerase activity in small samples of cell or tissue extracts. Due to the strict correlation between high levels of telomerase activity and neoplastic transformation, TRAP assay could provide an important diagnostic marker of malignancy. Although the original TRAP assay is very Sensitive and some improvements have been described, occasional artifacts still persist in the modified procedures. Here we describe how changes in the sequence of the primer used dor the amplification step enhance the reproducibility and sensitivity in the TRAP assay.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
