ABSTRACT Yolk proteins (vitellogenin and vitellin) proved to be excellent marker molecules for separating Anopheles gambiae Giles and An. arabiensis Patton, morphologically indistinguishable members of the An. gambiae species complex. A rabbit polyclonal antibody directed against An. gambiae yolk proteins made species-speci&c by removing immunoglobulins that crossreacted with An. arabiensis by immunoamnity chromatography. The resultant antibody 400 times sensitive An. gambiae and employed the secondary antibody in modified double antibody "sandwich" ELISA, which also used monoclonal antibodies anopheline vitellogenin the primary coating antibody. This ELISA easily differentiated soluble yolk protein samples from An. gambiae and An. biensis. A field study with 628 females of gambiae complex collected Kenya demonstrated that the ELISA results 98.4% in agreement with the standard method.
Differentiation of Anopheles gambiae and An.arabiensis (Diptera: Culicidae) by ELISA using immunoaffinity-purified antibodies to vitellogenin / Ma, M; Beier, Jc; Petrarca, Vincenzo; Gwadz, Rw; JIANG ZHONG, Shang; Qisheng, Song; Koech, Dk. - In: JOURNAL OF MEDICAL ENTOMOLOGY. - ISSN 0022-2585. - STAMPA. - 27:(1990), pp. 564-569.
Differentiation of Anopheles gambiae and An.arabiensis (Diptera: Culicidae) by ELISA using immunoaffinity-purified antibodies to vitellogenin.
PETRARCA, Vincenzo;
1990
Abstract
ABSTRACT Yolk proteins (vitellogenin and vitellin) proved to be excellent marker molecules for separating Anopheles gambiae Giles and An. arabiensis Patton, morphologically indistinguishable members of the An. gambiae species complex. A rabbit polyclonal antibody directed against An. gambiae yolk proteins made species-speci&c by removing immunoglobulins that crossreacted with An. arabiensis by immunoamnity chromatography. The resultant antibody 400 times sensitive An. gambiae and employed the secondary antibody in modified double antibody "sandwich" ELISA, which also used monoclonal antibodies anopheline vitellogenin the primary coating antibody. This ELISA easily differentiated soluble yolk protein samples from An. gambiae and An. biensis. A field study with 628 females of gambiae complex collected Kenya demonstrated that the ELISA results 98.4% in agreement with the standard method.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
