The globular proteinγB-crystallin exhibits a complex phase behavior, whereliquid−liquid phase separation characterized by a critical volume fractionφc= 0.154 and acritical temperatureTc= 291.8 K coexists with dynamical arrest on all length scales at volumefractions aroundφ≈0.3−0.35, and an arrest line that extends well into the unstable regionbelow the spinodal. However, although the static properties such as the osmoticcompressibility and the static correlation length are in quantitative agreement withpredictions for binary liquid mixtures, this is not the case for the dynamics of concentrationfluctuations described by the dynamic structure factorS(q,t). Using a combination ofdynamic light scattering and neutron spin echo measurements, we demonstrate that thecompetition between critical slowing down and dynamical arrest results in a much more complex wave vector dependence ofS(q,t) than previously anticipated

Unusual Dynamics of Concentration Fluctuations in Solutions of Weakly Attractive Globular Proteins / Bucciarelli, Saskia; Casal Dujat, Lucía; DE MICHELE, Cristiano; Sciortino, Francesco; Dhont, Jan; Bergenholtz, Johan; Farago, Bela; Schurtenberger, Peter; Stradner, Anna. - In: THE JOURNAL OF PHYSICAL CHEMISTRY LETTERS. - ISSN 1948-7185. - ELETTRONICO. - 6:22(2015), pp. 4470-4474. [10.1021/acs.jpclett.5b02092]

Unusual Dynamics of Concentration Fluctuations in Solutions of Weakly Attractive Globular Proteins

DE MICHELE, CRISTIANO;SCIORTINO, Francesco;
2015

Abstract

The globular proteinγB-crystallin exhibits a complex phase behavior, whereliquid−liquid phase separation characterized by a critical volume fractionφc= 0.154 and acritical temperatureTc= 291.8 K coexists with dynamical arrest on all length scales at volumefractions aroundφ≈0.3−0.35, and an arrest line that extends well into the unstable regionbelow the spinodal. However, although the static properties such as the osmoticcompressibility and the static correlation length are in quantitative agreement withpredictions for binary liquid mixtures, this is not the case for the dynamics of concentrationfluctuations described by the dynamic structure factorS(q,t). Using a combination ofdynamic light scattering and neutron spin echo measurements, we demonstrate that thecompetition between critical slowing down and dynamical arrest results in a much more complex wave vector dependence ofS(q,t) than previously anticipated
2015
arrest transition; critical phenomena; dynamic light scattering; lens proteins; neutron spin echo; protein dynamics; Materials Science (all)
01 Pubblicazione su rivista::01a Articolo in rivista
Unusual Dynamics of Concentration Fluctuations in Solutions of Weakly Attractive Globular Proteins / Bucciarelli, Saskia; Casal Dujat, Lucía; DE MICHELE, Cristiano; Sciortino, Francesco; Dhont, Jan; Bergenholtz, Johan; Farago, Bela; Schurtenberger, Peter; Stradner, Anna. - In: THE JOURNAL OF PHYSICAL CHEMISTRY LETTERS. - ISSN 1948-7185. - ELETTRONICO. - 6:22(2015), pp. 4470-4474. [10.1021/acs.jpclett.5b02092]
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11573/847540
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