The aim of the present study was to investigate the antioxidant activity and protective potential of T. tetraptera extracts against ion toxicity. The antioxidant activity of the extracts was investigated spectrophotometrically against several radicals (1,1-diphenyl-2-picrylhydrazyl (DPPH•), 2,2′-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) (ABTS•), hydroxyl radical (HO•), and nitric oxide (NO•)), followed by the ferric reducing power, total phenols, flavonoid, and flavonol contents. The effects of the extracts on catalase (CAT), superoxide dismutase (SOD), and peroxidase activities were also determined using the standard methods as well as the polyphenol profile using HPLC. The results showed that the hydroethanolic extract of T. tetraptera (CFH) has the lowest ICvalue with the DPPH, ABTS, OH, and NO radicals. The same extract also exhibited the significantly higher level of total phenols (37.24 ± 2.00 CAE/g dried extract); flavonoids (11.36 ± 1.88 QE/g dried extract); and flavonols contents (3.95 ± 0.39 QE/g dried extract). The HPLC profile of T. tetraptera revealed that eugenol (958.81 ± 00 mg/g DW), quercetin (353.78 ± 00 mg/g DW), and rutin (210.54 ± 00 mg/g DW) were higher in the fruit than the bark extracts. In conclusion, extracts from T. tetraptera may act as a protector against oxidative mediated ion toxicity. © 2015 Bruno Moukette Moukette et al.

In vitro ion chelating, antioxidative mechanism of extracts from fruits and barks of tetrapleura tetraptera and their protective effects against fenton mediated toxicity of metal ions on liver homogenates / Moukette, B. M.; Pieme, C. A.; Biapa, P. C. N.; Njimou, JACQUES ROMAIN; Stoller, Marco; Bravi, Marco; Ngogang, J. Y.. - In: EVIDENCE-BASED COMPLEMENTARY AND ALTERNATIVE MEDICINE. - ISSN 1741-427X. - ELETTRONICO. - 2015:(2015). [10.1155/2015/423689]

In vitro ion chelating, antioxidative mechanism of extracts from fruits and barks of tetrapleura tetraptera and their protective effects against fenton mediated toxicity of metal ions on liver homogenates

NJIMOU, JACQUES ROMAIN;Stoller, Marco;Bravi, Marco;
2015

Abstract

The aim of the present study was to investigate the antioxidant activity and protective potential of T. tetraptera extracts against ion toxicity. The antioxidant activity of the extracts was investigated spectrophotometrically against several radicals (1,1-diphenyl-2-picrylhydrazyl (DPPH•), 2,2′-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) (ABTS•), hydroxyl radical (HO•), and nitric oxide (NO•)), followed by the ferric reducing power, total phenols, flavonoid, and flavonol contents. The effects of the extracts on catalase (CAT), superoxide dismutase (SOD), and peroxidase activities were also determined using the standard methods as well as the polyphenol profile using HPLC. The results showed that the hydroethanolic extract of T. tetraptera (CFH) has the lowest ICvalue with the DPPH, ABTS, OH, and NO radicals. The same extract also exhibited the significantly higher level of total phenols (37.24 ± 2.00 CAE/g dried extract); flavonoids (11.36 ± 1.88 QE/g dried extract); and flavonols contents (3.95 ± 0.39 QE/g dried extract). The HPLC profile of T. tetraptera revealed that eugenol (958.81 ± 00 mg/g DW), quercetin (353.78 ± 00 mg/g DW), and rutin (210.54 ± 00 mg/g DW) were higher in the fruit than the bark extracts. In conclusion, extracts from T. tetraptera may act as a protector against oxidative mediated ion toxicity. © 2015 Bruno Moukette Moukette et al.
2015
1,1 diphenyl 2 picrylhydrazyl; 2,2' azinobis(3 ethylbenzothiazoline 6 sulfonic acid); catalase; eugenol; flavonoid; flavonol; hydroxyl radical; metal ion; nitric oxide; peroxidase; phenol derivative; plant extract; polyphenol; quercetin; rutoside; superoxide dismutase; Tetrapleura tetraptera extract; unclassified drug; animal experiment; animal tissue; antioxidant activity; article; bark; controlled study; enzyme activity; fruit; high performance liquid chromatography; IC50; in vitro study; lipid peroxidation; liver homogenate; liver protection; male; nonhuman; principal component analysis; priority journal; rat; spectrophotometry; Tetrapleura; Tetrapleura tetraptera
01 Pubblicazione su rivista::01a Articolo in rivista
In vitro ion chelating, antioxidative mechanism of extracts from fruits and barks of tetrapleura tetraptera and their protective effects against fenton mediated toxicity of metal ions on liver homogenates / Moukette, B. M.; Pieme, C. A.; Biapa, P. C. N.; Njimou, JACQUES ROMAIN; Stoller, Marco; Bravi, Marco; Ngogang, J. Y.. - In: EVIDENCE-BASED COMPLEMENTARY AND ALTERNATIVE MEDICINE. - ISSN 1741-427X. - ELETTRONICO. - 2015:(2015). [10.1155/2015/423689]
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11573/845285
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