Catanionic Vesicles Formed with Arginine-Based Surfactants and 1,2-Dipalmitoyl-sn-glycero-3-phosphate Monosodium Salt.

We report on mixing an anionic diacyl phospholipid (1,2-dipalmitoyl-sn-glycero-3-phosphate monosodium salt, DPPA) with either monoacyl and diacyl arginine-based surfactants. These mixtures are part of the rich family of pseudo-triple-chain and pseudo-tetra-chain catanionic mixtures, respectively. Vesicle size and _-potential were measured at several mixing ratios. Additional information on counterion binding, vesicle size, and integrity was obtained from ion selective electrode and Cryo-TEM measurements. Addition of positively charged surfactants to DPPA results in an increase of vesicle size. However, _-potential shows different trends, depending on whether water or acid media are used as solvent. In the latter, _-potential values progressively approach 0 upon addition of amino acid based surfactants. In water, surprisingly, _-potential values become more negative. The results are discussed in terms of modifications in counterion binding and vesicle size.