The pH regulation has a fundamental role in several intracellular processes and its variation via exogenous compounds is a potential tool for intervening in the intracellular processes. Proton caged compounds (PPCs) release protons upon UV irradiation and may efficiently provoke intracellular on-command acidification. Here, we explore the intracellular pH variation, when purposely synthesized PCCs are coupled to gold nanoparticles (AuNPs) and dosed to HEK-293 cells. We detected the acidification process caused by the UV irradiation by monitoring the intensity of the asymmetric stretching mode of the CO2 molecule at 2343 cm− 1. The comparison between free and AuNPs functionalized proton caged compound demonstrates a highly enhanced CO2 yield, hence pH variation, in the latter case. Finally, PCC functionalized AuNPs were marked with a purposely synthesized fluorescent marker and dosed to HEK-293 cells. The corresponding fluorescence optical images show green grains throughout the whole cytoplasm.

Exogenous control over intracellular acidification: Enhancement via proton caged compounds coupled to gold nanoparticles / Carbone, Marilena; Sabbatella, Gianfranco; Antonaroli, Simonetta; Remita, Hynd; Orlando, Viviana; Biagioni, Stefano; Nucara, Alessandro. - In: BIOCHIMICA ET BIOPHYSICA ACTA-GENERAL SUBJECTS. - ISSN 0304-4165. - STAMPA. - 1850:11(2015), pp. 2304-2307. [10.1016/j.bbagen.2015.07.011]

Exogenous control over intracellular acidification: Enhancement via proton caged compounds coupled to gold nanoparticles

SABBATELLA, GIANFRANCO;ORLANDO, Viviana;BIAGIONI, Stefano;NUCARA, Alessandro
2015

Abstract

The pH regulation has a fundamental role in several intracellular processes and its variation via exogenous compounds is a potential tool for intervening in the intracellular processes. Proton caged compounds (PPCs) release protons upon UV irradiation and may efficiently provoke intracellular on-command acidification. Here, we explore the intracellular pH variation, when purposely synthesized PCCs are coupled to gold nanoparticles (AuNPs) and dosed to HEK-293 cells. We detected the acidification process caused by the UV irradiation by monitoring the intensity of the asymmetric stretching mode of the CO2 molecule at 2343 cm− 1. The comparison between free and AuNPs functionalized proton caged compound demonstrates a highly enhanced CO2 yield, hence pH variation, in the latter case. Finally, PCC functionalized AuNPs were marked with a purposely synthesized fluorescent marker and dosed to HEK-293 cells. The corresponding fluorescence optical images show green grains throughout the whole cytoplasm.
2015
Gold nanoparticles; Intracellular acidification; pH monitoring; Proton caged compounds; Biochemistry; Biophysics; Molecular Biology
01 Pubblicazione su rivista::01a Articolo in rivista
Exogenous control over intracellular acidification: Enhancement via proton caged compounds coupled to gold nanoparticles / Carbone, Marilena; Sabbatella, Gianfranco; Antonaroli, Simonetta; Remita, Hynd; Orlando, Viviana; Biagioni, Stefano; Nucara, Alessandro. - In: BIOCHIMICA ET BIOPHYSICA ACTA-GENERAL SUBJECTS. - ISSN 0304-4165. - STAMPA. - 1850:11(2015), pp. 2304-2307. [10.1016/j.bbagen.2015.07.011]
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11573/834039
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