The analysis of Y chromosome short tandem repeat (Y-STR) haplotypes provides important information that can be used for investigative purposes and in population studies. The Yfiler® Plus PCR Amplification kit (Yfiler® Plus, Thermo Fisher Scientific, Waltham, MA, USA) allows the multiplex amplification of 27 Y-STRs, including 7 rapidly mutating markers (RM Y-STRs). In this study, 203 unrelated males from Italy, which were subdivided into 4 different geographical groups (North, Center, South and Sardinia) were analyzed. Several intra-population diversity indexes were computed and compared to those obtained using only loci either from the minimal haplotype or the 17-plex (Yfiler®, Thermo Fisher Scientific, Waltham, MA, USA). In addition, inter-population diversity analysis (RST) among the four Italian samples was performed. The same analysis was also used to compare the Italian sub-sets to other European populations where the Yfiler® Plus haplotype frequency data were available. The Sardinians were significantly differentiated from the other three Italian groups, thus requiring a specific sub-national Y-STR haplotype database. The Yfiler® Plus kit showed a high power of discrimination which is useful for criminal investigations, principally due to the inclusion of RM Y-STRs.

Forensic genetic value of a 27 Y-STR loci multiplex (Yfiler® Plus kit) in an Italian population sample / Rapone, Cesare; D'Atanasio, Eugenia; Agostino, Alessandro; Mariano, Martina; Papaluca, Maria Teresa; Cruciani, Fulvio; Berti, Andrea. - In: FORENSIC SCIENCE INTERNATIONAL: GENETICS. - ISSN 1872-4973. - ELETTRONICO. - 21:(2016), pp. e1-e5. [10.1016/j.fsigen.2015.11.006]

Forensic genetic value of a 27 Y-STR loci multiplex (Yfiler® Plus kit) in an Italian population sample

D'ATANASIO, EUGENIA;CRUCIANI, Fulvio;
2016

Abstract

The analysis of Y chromosome short tandem repeat (Y-STR) haplotypes provides important information that can be used for investigative purposes and in population studies. The Yfiler® Plus PCR Amplification kit (Yfiler® Plus, Thermo Fisher Scientific, Waltham, MA, USA) allows the multiplex amplification of 27 Y-STRs, including 7 rapidly mutating markers (RM Y-STRs). In this study, 203 unrelated males from Italy, which were subdivided into 4 different geographical groups (North, Center, South and Sardinia) were analyzed. Several intra-population diversity indexes were computed and compared to those obtained using only loci either from the minimal haplotype or the 17-plex (Yfiler®, Thermo Fisher Scientific, Waltham, MA, USA). In addition, inter-population diversity analysis (RST) among the four Italian samples was performed. The same analysis was also used to compare the Italian sub-sets to other European populations where the Yfiler® Plus haplotype frequency data were available. The Sardinians were significantly differentiated from the other three Italian groups, thus requiring a specific sub-national Y-STR haplotype database. The Yfiler® Plus kit showed a high power of discrimination which is useful for criminal investigations, principally due to the inclusion of RM Y-STRs.
2016
Y chromosome, forensic genetics, microsatellites, RM Y-STR
01 Pubblicazione su rivista::01a Articolo in rivista
Forensic genetic value of a 27 Y-STR loci multiplex (Yfiler® Plus kit) in an Italian population sample / Rapone, Cesare; D'Atanasio, Eugenia; Agostino, Alessandro; Mariano, Martina; Papaluca, Maria Teresa; Cruciani, Fulvio; Berti, Andrea. - In: FORENSIC SCIENCE INTERNATIONAL: GENETICS. - ISSN 1872-4973. - ELETTRONICO. - 21:(2016), pp. e1-e5. [10.1016/j.fsigen.2015.11.006]
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