Cullin-RING E3 ligases (CRLs) regulate different aspects of plant development, and are activated by modification of their cullin subunit with the ubiquitin-like protein NEDD8 (NEural precursor cell expressed Developmentally Down-regulated 8) (neddylation) and deactivated by NEDD8 removal (deneddylation). The CONSTITUTIVELY PHOTOMORPHOGENIC9 (COP9) signalosome (CSN) acts as a molecular switch of CRLs activity by reverting their neddylation status, but its contribution to embryonic and early seedling development remains poorly characterized. Here, we analyzed the phenotypic defects of csn mutants and monitored the cullin deneddylation/neddylation ratio during embryonic and early seedling development. We show that while csn mutants can complete embryogenesis (albeit at a slower pace than wild type) and are able to germinate (albeit at a reduced rate), they progressively loose meristem activity upon germination, until they become unable to sustain growth. We also show that the majority of cullin proteins is progressively neddylated during the late stages of seed maturation and becomes deneddylated upon seed germination. This developmentally regulated shift in the cullin neddylation status is absent in csn mutants. We conclude that the CSN and its cullin deneddylation activity are required to sustain postembryonic meristem function in Arabidopsis.

The COP9 SIGNALOSOME is required for postembryonic meristem maintenance in Arabidopsis thaliana / Franciosini, Anna; Moubayidin, Laila; Du, Kaiqi; Matari, Nahill H; Boccaccini, Alessandra; Butera, Simone; Vittorioso, Paola; Sabatini, Sabrina; Jenik, Pablo D; Costantino, Paolo; Serino, Giovanna. - In: MOLECULAR PLANT. - ISSN 1674-2052. - STAMPA. - 8:11(2015), pp. 1623-1634. [10.1016/j.molp.2015.08.003]

The COP9 SIGNALOSOME is required for postembryonic meristem maintenance in Arabidopsis thaliana

FRANCIOSINI, ANNA;MOUBAYIDIN, LAILA;BOCCACCINI, ALESSANDRA;VITTORIOSO, Paola;SABATINI, Sabrina;COSTANTINO, Paolo;SERINO, Giovanna
2015

Abstract

Cullin-RING E3 ligases (CRLs) regulate different aspects of plant development, and are activated by modification of their cullin subunit with the ubiquitin-like protein NEDD8 (NEural precursor cell expressed Developmentally Down-regulated 8) (neddylation) and deactivated by NEDD8 removal (deneddylation). The CONSTITUTIVELY PHOTOMORPHOGENIC9 (COP9) signalosome (CSN) acts as a molecular switch of CRLs activity by reverting their neddylation status, but its contribution to embryonic and early seedling development remains poorly characterized. Here, we analyzed the phenotypic defects of csn mutants and monitored the cullin deneddylation/neddylation ratio during embryonic and early seedling development. We show that while csn mutants can complete embryogenesis (albeit at a slower pace than wild type) and are able to germinate (albeit at a reduced rate), they progressively loose meristem activity upon germination, until they become unable to sustain growth. We also show that the majority of cullin proteins is progressively neddylated during the late stages of seed maturation and becomes deneddylated upon seed germination. This developmentally regulated shift in the cullin neddylation status is absent in csn mutants. We conclude that the CSN and its cullin deneddylation activity are required to sustain postembryonic meristem function in Arabidopsis.
Arabidopsis thaliana; COP9 signalosome; Cullin-Ring ubiquitin ligase; embryo development; meristem; seed maturation; ubiquitin
01 Pubblicazione su rivista::01a Articolo in rivista
The COP9 SIGNALOSOME is required for postembryonic meristem maintenance in Arabidopsis thaliana / Franciosini, Anna; Moubayidin, Laila; Du, Kaiqi; Matari, Nahill H; Boccaccini, Alessandra; Butera, Simone; Vittorioso, Paola; Sabatini, Sabrina; Jenik, Pablo D; Costantino, Paolo; Serino, Giovanna. - In: MOLECULAR PLANT. - ISSN 1674-2052. - STAMPA. - 8:11(2015), pp. 1623-1634. [10.1016/j.molp.2015.08.003]
File allegati a questo prodotto
File Dimensione Formato  
Franciosini_COP9_2015.pdf

accesso aperto

Note: articolo scientifico
Tipologia: Versione editoriale (versione pubblicata con il layout dell'editore)
Licenza: Tutti i diritti riservati (All rights reserved)
Dimensione 2.52 MB
Formato Adobe PDF
2.52 MB Adobe PDF Visualizza/Apri PDF

I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.

Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11573/831248
Citazioni
  • ???jsp.display-item.citation.pmc??? 9
  • Scopus 16
  • ???jsp.display-item.citation.isi??? 14
social impact