In label-free biosensing, a continuous improvement of the limit of detection is necessary to resolve the small change of the surface refractive index produced by interacting biomolecules at a very small concentration. In the present work, optical sensors based on one-dimensional photonic crystals supporting Bloch surface waves are proposed and adopted for label-free optical biosensing. We describe the implementation of an angularly resolved ellipsometric optical sensing scheme based on Bloch surface waves sustained by tantala/silica multilayers. The angular operation is obtained using a focused beam at fixed wavelength and detection of the angular reflectance spectrum by means of an array detector. The results show that the experimental limit of detection for a particular photonic crystal design is 6.5×10−7 refractive index units (RIU)/Hz1/2 and further decrease could be obtained. For the first time, we report on the practical application of this technique to a cancer biomarker protocol that aims at the detection of a specific glycoprotein (angiopoietin 2) involved in angiogenesis and inflammation processes.

Angularly resolved ellipsometric optical biosensing by means of Bloch surface waves / Sinibaldi, Alberto; Anopchenko, A.; Rizzo, R.; Danz, N.; Munzert, P.; Rivolo, P.; Frascella, F.; Ricciardi, S.; Michelotti, Francesco. - In: ANALYTICAL AND BIOANALYTICAL CHEMISTRY. - ISSN 1618-2642. - STAMPA. - 407:(2015), pp. 3965-3974. [10.1007/s00216-015-8591-8]

Angularly resolved ellipsometric optical biosensing by means of Bloch surface waves

SINIBALDI, ALBERTO;MICHELOTTI, Francesco
2015

Abstract

In label-free biosensing, a continuous improvement of the limit of detection is necessary to resolve the small change of the surface refractive index produced by interacting biomolecules at a very small concentration. In the present work, optical sensors based on one-dimensional photonic crystals supporting Bloch surface waves are proposed and adopted for label-free optical biosensing. We describe the implementation of an angularly resolved ellipsometric optical sensing scheme based on Bloch surface waves sustained by tantala/silica multilayers. The angular operation is obtained using a focused beam at fixed wavelength and detection of the angular reflectance spectrum by means of an array detector. The results show that the experimental limit of detection for a particular photonic crystal design is 6.5×10−7 refractive index units (RIU)/Hz1/2 and further decrease could be obtained. For the first time, we report on the practical application of this technique to a cancer biomarker protocol that aims at the detection of a specific glycoprotein (angiopoietin 2) involved in angiogenesis and inflammation processes.
Optical biosensing, Label-free biosensors, Photonic crystals, Bloch surface waves, Ellipsometry, Biophotonics
01 Pubblicazione su rivista::01a Articolo in rivista
Angularly resolved ellipsometric optical biosensing by means of Bloch surface waves / Sinibaldi, Alberto; Anopchenko, A.; Rizzo, R.; Danz, N.; Munzert, P.; Rivolo, P.; Frascella, F.; Ricciardi, S.; Michelotti, Francesco. - In: ANALYTICAL AND BIOANALYTICAL CHEMISTRY. - ISSN 1618-2642. - STAMPA. - 407:(2015), pp. 3965-3974. [10.1007/s00216-015-8591-8]
File allegati a questo prodotto
File Dimensione Formato  
Sinibaldi_Angularly_2015.pdf

accesso aperto

Tipologia: Documento in Post-print (versione successiva alla peer review e accettata per la pubblicazione)
Licenza: Creative commons
Dimensione 981.63 kB
Formato Adobe PDF
981.63 kB Adobe PDF Visualizza/Apri PDF

I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.

Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11573/817249
Citazioni
  • ???jsp.display-item.citation.pmc??? ND
  • Scopus 21
  • ???jsp.display-item.citation.isi??? 18
social impact