Given the pivotal role of monocyte-derived dendritic cells (DCs) in determining the magnitude of the antiviral innate immune response, we sought to determine whether Usutu virus (USUV) and West Nile virus (WNV) lineages (L)1 and L2 can infect DCs and affect the rate of type I interferon (IFN) activation. The sensitivity of these viruses to types I and III IFNs was also compared. We found that USUV can infect DCs, induce higher antiviral activities, IFN alpha subtypes and the IFN stimulated gene (ISG)15 pathway, and is more sensitive to types I and III IFNs than WNVs. In contrast, we confirmed that IFN alpha/beta subtypes were more effective against WNV L2 than WNV L1. However, the replication kinetics, induction of IFN alpha subtypes and ISGs in DCs and the sensitivity to IFN lambda 1-3 did not differ between WNV L1 and L2.

Variation in interferon sensitivity and induction between Usutu and West Nile (lineages 1 and 2) viruses / Cacciotti, Giulia; Caputo, Beniamino; Selvaggi, Carla; la Sala, Andrea; Vitiello, Laura; Diallo, Diawo; Ceianu, Cornelia; Antonelli, Guido; Nowotny, Norbert; Scagnolari, Carolina. - In: VIROLOGY. - ISSN 1096-0341. - STAMPA. - 485:(2015), pp. 189-198. [10.1016/j.virol.2015.07.015]

Variation in interferon sensitivity and induction between Usutu and West Nile (lineages 1 and 2) viruses

CACCIOTTI, GIULIA;CAPUTO, BENIAMINO;SELVAGGI, CARLA;ANTONELLI, Guido;SCAGNOLARI, CAROLINA
2015

Abstract

Given the pivotal role of monocyte-derived dendritic cells (DCs) in determining the magnitude of the antiviral innate immune response, we sought to determine whether Usutu virus (USUV) and West Nile virus (WNV) lineages (L)1 and L2 can infect DCs and affect the rate of type I interferon (IFN) activation. The sensitivity of these viruses to types I and III IFNs was also compared. We found that USUV can infect DCs, induce higher antiviral activities, IFN alpha subtypes and the IFN stimulated gene (ISG)15 pathway, and is more sensitive to types I and III IFNs than WNVs. In contrast, we confirmed that IFN alpha/beta subtypes were more effective against WNV L2 than WNV L1. However, the replication kinetics, induction of IFN alpha subtypes and ISGs in DCs and the sensitivity to IFN lambda 1-3 did not differ between WNV L1 and L2.
2015
IFN; interferon; monocyte-derived dendritic cells; USUV; Usutu virus; WNV; west Nile virus
01 Pubblicazione su rivista::01a Articolo in rivista
Variation in interferon sensitivity and induction between Usutu and West Nile (lineages 1 and 2) viruses / Cacciotti, Giulia; Caputo, Beniamino; Selvaggi, Carla; la Sala, Andrea; Vitiello, Laura; Diallo, Diawo; Ceianu, Cornelia; Antonelli, Guido; Nowotny, Norbert; Scagnolari, Carolina. - In: VIROLOGY. - ISSN 1096-0341. - STAMPA. - 485:(2015), pp. 189-198. [10.1016/j.virol.2015.07.015]
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11573/816210
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