In Saccharomyces cerevisiae most mitochondrial tRNA genes are clustered in a 9 kbp region between the cap and oxil genes. Polygenic transcripts of this region have been previously identified. A transcriptional initiation site at a TTATAAGTA box, located upstream from the tRNAcys gene, has now been detected by SI mapping experiments and by the capping of primary transcripts. Results are consistent with the hypothesis that this box represents the initiation site for transcription of a cluster of tRNA genes, while the adjacent tRNAthr is cotranscribed with the 21S rRNA. Results obtained with various strains are compared, and the efficiency of this sequence as a tran scriptional initiation site in different mitochondrial contexts is discussed.

Initiation of tran-scription of Mitochondrial tRNA gene cluster in S.cerevisiae

PALLESCHI, Claudio;FRANCISCI, Silvia;BIANCHI, Michele Maria;FRONTALI, Laura
1984

Abstract

In Saccharomyces cerevisiae most mitochondrial tRNA genes are clustered in a 9 kbp region between the cap and oxil genes. Polygenic transcripts of this region have been previously identified. A transcriptional initiation site at a TTATAAGTA box, located upstream from the tRNAcys gene, has now been detected by SI mapping experiments and by the capping of primary transcripts. Results are consistent with the hypothesis that this box represents the initiation site for transcription of a cluster of tRNA genes, while the adjacent tRNAthr is cotranscribed with the 21S rRNA. Results obtained with various strains are compared, and the efficiency of this sequence as a tran scriptional initiation site in different mitochondrial contexts is discussed.
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Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/11573/79939
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