Detection of viral nucleic acids in biological samples is most often based on molecular amplification followed by sequence-specific detection of amplicons. Isothermal nucleic acid amplification strategies are particularly suited for their implementation in lab-on-chip devices, owing to the simplicity of the protocol and instrumentation required. Among optical detection strategies, chemiluminescence offers the advantages of high detectability in low volumes and no requirements for lamps, filters, or specific optical arrangements.
Detection of viral DNA by isothermal NASBA amplification and chemiluminescence gene probe hybridization assay in a microfluidic cartridge / Bonvicini, F.; Mirasoli, M.; Zangheri, M.; Nascetti, Augusto; DE CESARE, Giampiero; Caputo, Domenico; Roda, A.; Gallinella, G.. - In: JOURNAL OF CLINICAL VIROLOGY. - ISSN 1386-6532. - STAMPA. - 70:(2015), pp. S91-S92. [10.1016/j.jcv.2015.07.213]
Detection of viral DNA by isothermal NASBA amplification and chemiluminescence gene probe hybridization assay in a microfluidic cartridge
NASCETTI, Augusto;DE CESARE, Giampiero;CAPUTO, Domenico;
2015
Abstract
Detection of viral nucleic acids in biological samples is most often based on molecular amplification followed by sequence-specific detection of amplicons. Isothermal nucleic acid amplification strategies are particularly suited for their implementation in lab-on-chip devices, owing to the simplicity of the protocol and instrumentation required. Among optical detection strategies, chemiluminescence offers the advantages of high detectability in low volumes and no requirements for lamps, filters, or specific optical arrangements.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
