Microfluidic chips with integrated amorphous silicon sensors for point-of-care testing

Herein we present a point-of-care device for immunodiagnostic tests. This device integrates, on the same glass substrate, a PDMS microfluidic network, an array of amorphous silicon photosensors for on-chip optical detection and a dedicated surface chemistry based on polymer brushes. As proof of principle, a peptide named VEA was immobilized on PHEMA polymer brushes. The survey relies on the formation, inside the microchannels, of a sandwich between primary antibody against VEA and a secondary antibody labeled with HRP, which catalyzes the reaction between luminol and hydrogen peroxide, yielding a chemiluminescent signal detected by the array of photosensors deposited underneath.