An inflamed human intestinal cell culture model to study the protective effects of plant-derived foodsAn inflamed human intestinal cell culture model to study the protective effects of plant-derived foodsThe human intestinal Caco-2 cell line grown and differentiated on permeable filter supports is an established model of the gut barrier and is extensively used to study the absorption, metabolism and toxicity of drugs and of substances ingested with the diet. When subjected to an inflammatory stimulus, by inducing marginal zinc deficiency and treating with Tumour Necrosis Factor α, Caco-2 cells respond with an increase in cell monolayer permeability and initiate a zinc-dependent survival pathway involving modulation of transcription and translation of Inhibitor of Apoptosis Proteins and NFkB activation. This experimental model has been applied to the study of potentially beneficial effects of plant-derived foods to human health. Brassica oleracea (broccoli) sprouts were grown under conditions known to enhance their content in bioactive molecules, such as glucosinolates and phenolic compounds, that are believed to be involved in the health protective effects associated with their consumption. A pipeline process has been developed from the controlled growth of broccoli sprouts, to the production of juices, their compositional analysis by LC-MS metabolomics and the assessment of their biological effects on the inflamed Caco-2 cell model. Broccoli sprout juices showed a protective effect from the inflammatory stimulus, by reducing the decline in trans epithelial electrical resistance, an index of decreased function of the gut barrier. Moreover, stronger protection was observed with juices grown under inductive conditions (light+osmotic stress) than from those grown under basal conditions (dark). Comparative analysis of the juices showed that the composition in bioactive molecules, such as anthocyanins and polyphenols, was associated with the differences in the observed protective effect. This study shows that the combination of metabolic profiling and biological testing with appropriate chemometric analysis is a valuable approach for studying cellular responses to different plant-derived foods and to functional food enrichment.
An inflamed human intestinal cell culture model to study the protective effects of plant-derived foodsAn inflamed human intestinal cell culture model to study the protective effects of plant-derived foodsThe human intestinal Caco-2 cell line grown and differentiated on permeable filter supports is an established model of the gut barrier and is extensively used to study the absorption, metabolism and toxicity of drugs and of substances ingested with the diet. When subjected to an inflammatory stimulus, by inducing marginal zinc deficiency and treating with Tumour Necrosis Factor α, Caco-2 cells respond with an increase in cell monolayer permeability and initiate a zinc-dependent survival pathway involving modulation of transcription and translation of Inhibitor of Apoptosis Proteins and NFkB activation. This experimental model has been applied to the study of potentially beneficial effects of plant-derived foods to human health. Brassica oleracea (broccoli) sprouts were grown under conditions known to enhance their content in bioactive molecules, such as glucosinolates and phenolic compounds, that are believed to be involved in the health protective effects associated with their consumption. A pipeline process has been developed from the controlled growth of broccoli sprouts, to the production of juices, their compositional analysis by LC-MS metabolomics and the assessment of their biological effects on the inflamed Caco-2 cell model. Broccoli sprout juices showed a protective effect from the inflammatory stimulus, by reducing the decline in trans epithelial electrical resistance, an index of decreased function of the gut barrier. Moreover, stronger protection was observed with juices grown under inductive conditions (light+osmotic stress) than from those grown under basal conditions (dark). Comparative analysis of the juices showed that the composition in bioactive molecules, such as anthocyanins and polyphenols, was associated with the differences in the observed protective effect. This study shows that the combination of metabolic profiling and biological testing with appropriate chemometric analysis is a valuable approach for studying cellular responses to different plant-derived foods and to functional food enrichment.
An inflamed human intestinal cell culture model to study the protective effects of plant-derived foods / Yula, Sambuy; Simonetta, Ferruzza; Giusti, Anna Maria; Marialuce, Maldini; Fulvio, Mattivi; Elisabetta, Moneta; Morelli, Giorgio; Murgia, MARIA CHIARA; Mirella, Nardini; Fausta, Natella; Giulia, Ranaldi; Carlotta, Rossi; Cristina, Scaccini; Kajetan, Trost; Simona, Baima. - STAMPA. - (2015), pp. 13-14. (Intervento presentato al convegno 7th Annual kirkstall conference, Advances in cell and tissue cultur tenutosi a Pisa nel 15th-17th June 2015).
An inflamed human intestinal cell culture model to study the protective effects of plant-derived foods
GIUSTI, Anna Maria;MORELLI, GIORGIO;MURGIA, MARIA CHIARA;
2015
Abstract
An inflamed human intestinal cell culture model to study the protective effects of plant-derived foodsAn inflamed human intestinal cell culture model to study the protective effects of plant-derived foodsThe human intestinal Caco-2 cell line grown and differentiated on permeable filter supports is an established model of the gut barrier and is extensively used to study the absorption, metabolism and toxicity of drugs and of substances ingested with the diet. When subjected to an inflammatory stimulus, by inducing marginal zinc deficiency and treating with Tumour Necrosis Factor α, Caco-2 cells respond with an increase in cell monolayer permeability and initiate a zinc-dependent survival pathway involving modulation of transcription and translation of Inhibitor of Apoptosis Proteins and NFkB activation. This experimental model has been applied to the study of potentially beneficial effects of plant-derived foods to human health. Brassica oleracea (broccoli) sprouts were grown under conditions known to enhance their content in bioactive molecules, such as glucosinolates and phenolic compounds, that are believed to be involved in the health protective effects associated with their consumption. A pipeline process has been developed from the controlled growth of broccoli sprouts, to the production of juices, their compositional analysis by LC-MS metabolomics and the assessment of their biological effects on the inflamed Caco-2 cell model. Broccoli sprout juices showed a protective effect from the inflammatory stimulus, by reducing the decline in trans epithelial electrical resistance, an index of decreased function of the gut barrier. Moreover, stronger protection was observed with juices grown under inductive conditions (light+osmotic stress) than from those grown under basal conditions (dark). Comparative analysis of the juices showed that the composition in bioactive molecules, such as anthocyanins and polyphenols, was associated with the differences in the observed protective effect. This study shows that the combination of metabolic profiling and biological testing with appropriate chemometric analysis is a valuable approach for studying cellular responses to different plant-derived foods and to functional food enrichment.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
