In this paper, we present an alternative fluorochrome labeled DNA detection system based on the use of an amorphous silicon photosensor. The sensor is an n-i-p stacked structure whose photo-response has been optimized by using a numerical device simulator. The optimization process was performed as a trade off between the spectral selectivity and the dark current of the device in order to increase the signal-to-noise ratio. It has been observed that the system detection limit lies around 3 nmol/l. Taking into account the specific activity of labeled DNA in the solution, it corresponds to a minimal surface density of fluorochrome around 50 fmol/cm2. This result is very encouraging because it is obtained with a low cost system, where the use of optics for focusing and filtering of both exciting and emitting radiation is avoided.
Detection of labelled DNA based on amorphous silicon devices / Caputo, Domenico; DE CESARE, Giampiero; Nascetti, Augusto; Scipinotti, Riccardo; Negri, Rodolfo; A., Tarquini. - STAMPA. - (2006), pp. 182-186. (Intervento presentato al convegno 11th Italian Conference on Sensor and Microsystems tenutosi a Lecce - Italy nel 8-10 February 2006).
Detection of labelled DNA based on amorphous silicon devices
CAPUTO, Domenico;DE CESARE, Giampiero;NASCETTI, Augusto;SCIPINOTTI, RICCARDO;NEGRI, RODOLFO;
2006
Abstract
In this paper, we present an alternative fluorochrome labeled DNA detection system based on the use of an amorphous silicon photosensor. The sensor is an n-i-p stacked structure whose photo-response has been optimized by using a numerical device simulator. The optimization process was performed as a trade off between the spectral selectivity and the dark current of the device in order to increase the signal-to-noise ratio. It has been observed that the system detection limit lies around 3 nmol/l. Taking into account the specific activity of labeled DNA in the solution, it corresponds to a minimal surface density of fluorochrome around 50 fmol/cm2. This result is very encouraging because it is obtained with a low cost system, where the use of optics for focusing and filtering of both exciting and emitting radiation is avoided.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
