A rapid and sensitive CZE (Capillary Zone Electrophoresis) method for pharmaceutical analysis was developed and fully validated. The active compounds : Pseudoephedrine hydrochloride ( PSE), Triprolidine hydrochloride (TRI) and Paracetamol ( PAR) were separated and quantitatively determined using the tris–borate 30mM buffer at pH = 9.0 as a Background Electrolyte (BGE).The electrophoretic separation was carried out at 25 kV in an unmodified fused silica capillary of I.D. = 50 µm with a “ bubble-cell” for UV detection at 210nm and 25°C.The separation was reached in about 3 min. After calibration the method was applied for analysis of three commercially available pharmaceutical preparations.. The repeatability (RSD%) of migration time (tm) was ranging between 0.47% and 0.90% and of peak areas (A) between 0.63% and 3.64%. The Limit of Detection (LOD) values were of 0.19 μg/mL, 0.31 μg/mL and 0.08 μg/mL for respectively PSE, TRI and PAR. The results obtained in this study showed that the proposed method is useful in routinely analysis of pharmaceuticals.

Rapid and sensitive CZE method for Quality Control analysis of pharmaceuticals containing Pseudoephedrine, Triprolidine and Paracetamol / S., Di Berardino; Jasionowska, Renata. - In: AMERICAN JOURNAL OF ANALYTICAL CHEMISTRY. - ISSN 2156-8251. - STAMPA. - 5(9):(2014), pp. 613-619. [10.4236/ajac.2014.59069]

Rapid and sensitive CZE method for Quality Control analysis of pharmaceuticals containing Pseudoephedrine, Triprolidine and Paracetamol.

JASIONOWSKA, Renata
2014

Abstract

A rapid and sensitive CZE (Capillary Zone Electrophoresis) method for pharmaceutical analysis was developed and fully validated. The active compounds : Pseudoephedrine hydrochloride ( PSE), Triprolidine hydrochloride (TRI) and Paracetamol ( PAR) were separated and quantitatively determined using the tris–borate 30mM buffer at pH = 9.0 as a Background Electrolyte (BGE).The electrophoretic separation was carried out at 25 kV in an unmodified fused silica capillary of I.D. = 50 µm with a “ bubble-cell” for UV detection at 210nm and 25°C.The separation was reached in about 3 min. After calibration the method was applied for analysis of three commercially available pharmaceutical preparations.. The repeatability (RSD%) of migration time (tm) was ranging between 0.47% and 0.90% and of peak areas (A) between 0.63% and 3.64%. The Limit of Detection (LOD) values were of 0.19 μg/mL, 0.31 μg/mL and 0.08 μg/mL for respectively PSE, TRI and PAR. The results obtained in this study showed that the proposed method is useful in routinely analysis of pharmaceuticals.
2014
pseudoephedrine; paracetamol; triprolidine hydrochloride; capillary zone electrophoresis; quality control
01 Pubblicazione su rivista::01a Articolo in rivista
Rapid and sensitive CZE method for Quality Control analysis of pharmaceuticals containing Pseudoephedrine, Triprolidine and Paracetamol / S., Di Berardino; Jasionowska, Renata. - In: AMERICAN JOURNAL OF ANALYTICAL CHEMISTRY. - ISSN 2156-8251. - STAMPA. - 5(9):(2014), pp. 613-619. [10.4236/ajac.2014.59069]
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11573/784103
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