Rapid and sensitive CZE method for Quality Control analysis of pharmaceuticals containing Pseudoephedrine, Triprolidine and Paracetamol.

A rapid and sensitive CZE (Capillary Zone Electrophoresis) method for pharmaceutical analysis was developed and fully validated. The active compounds : Pseudoephedrine hydrochloride ( PSE), Triprolidine hydrochloride (TRI) and Paracetamol ( PAR) were separated and quantitatively determined using the tris–borate 30mM buffer at pH = 9.0 as a Background Electrolyte (BGE).The electrophoretic separation was carried out at 25 kV in an unmodified fused silica capillary of I.D. = 50 µm with a “ bubble-cell” for UV detection at 210nm and 25°C.The separation was reached in about 3 min. After calibration the method was applied for analysis of three commercially available pharmaceutical preparations.. The repeatability (RSD%) of migration time (tm) was ranging between 0.47% and 0.90% and of peak areas (A) between 0.63% and 3.64%. The Limit of Detection (LOD) values were of 0.19 μg/mL, 0.31 μg/mL and 0.08 μg/mL for respectively PSE, TRI and PAR. The results obtained in this study showed that the proposed method is useful in routinely analysis of pharmaceuticals.