The investigation of possible protein-unfolding effects induced by high intensity electric field pulses is becoming extremely interesting also due to the increasing use of nanopulses applications. In this context an integrated approach combining experimental biochemical endpoints with numerical modeling based on molecular dynamics is challenging. In this work experimental results on nanopulses applied field applied to the superoxide dismutase enzyme have been interpreted by means of molecular dynamics simulations used as a tool to support hypothesis on possible protein unfolding mediated by high intense electric fields.
Pulsed E fields acting on metallo-enzymes: theoretical and experimental results / DELLA VALLE, Elena; Olga, Pakhomova; Marracino, Paolo; Shu, Xiao; Andrei, Pakhomov; Liberti, Micaela; Apollonio, Francesca. - (2014). (Intervento presentato al convegno BioEM2014 Joint Meeting of The Bioelectromagnetics Society and the European BioElectromagnetics Association tenutosi a Cape Town, South Africa nel 8 - 13 June, 2014).
Pulsed E fields acting on metallo-enzymes: theoretical and experimental results
DELLA VALLE, ELENA;MARRACINO, PAOLO;LIBERTI, Micaela;APOLLONIO, Francesca
2014
Abstract
The investigation of possible protein-unfolding effects induced by high intensity electric field pulses is becoming extremely interesting also due to the increasing use of nanopulses applications. In this context an integrated approach combining experimental biochemical endpoints with numerical modeling based on molecular dynamics is challenging. In this work experimental results on nanopulses applied field applied to the superoxide dismutase enzyme have been interpreted by means of molecular dynamics simulations used as a tool to support hypothesis on possible protein unfolding mediated by high intense electric fields.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
