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EnglishOBJECTIVES: To investigate the expression of IL-36 axis in patients with primary Sjogren's syndrome (pSS). METHODS: Blood and minor labial salivary glands (MSG) biopsies were obtained from 35 pSS and 20 nSS patients. Serum IL-36α was assayed by ELISA. IL-36α, IL-36R, IL-36RA, IL-38, IL-22, IL-17, IL-23p19, expression in MSGs was assessed by rt-PCR and tissue IL-36α and IL-38 expression also investigated by immuno-histochemistry (IHC). αβ and γδ T cells and CD68+ cells isolated from MSGs were also studied by flow cytometry and confocal microscopy analysis. RESULTS: IL-36α was significantly over-expressed in the serum and in the salivary glands of pSS. Salivary gland IL-36α expression was correlated with the expression levels of IL-17, IL-22 and IL-23p19. IL-38 that acts as inhibitor of IL-36α was also up-regulated in pSS. αβ+ CD3+ T cells and CD68+ cells were the major source of IL-36α in minor salivary glands of pSS. γδ T cells were not significantly expanded in the salivary glands of pSS but produced more IL-17 being their percentage correlated with the focus score. Higher expression of IL-36α and IL-36R was also demonstrated in γδ T cells isolated from pSS compared to controls. CONCLUSIONS: in this study we demonstrate that a significant increase in circulating and tissue levels of IL-36α occurs in pSS patients.
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| Titolo: | Interleukin-36α axis is modulated in patients with primary Sjögren's syndrome. |
| Autori: | |
| Data di pubblicazione: | 2015 |
| Rivista: | |
| Handle: | http://hdl.handle.net/11573/782988 |
| Appartiene alla tipologia: | 01a Articolo in rivista |
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| File | Note | Tipologia | Licenza | |
|---|---|---|---|---|
| Ciccia_Interleukin-36 a_2015.pdf | Documento in Post-print (versione successiva alla peer review e accettata per la pubblicazione) | Tutti i diritti riservati (All rights reserved) | Open Access Visualizza/Apri |
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