In this work we present an integrated biosensor that enables FTIR (Fourier Transform-Infrared) detection of analytes contained in diluted solutions. The fabricated nanosensor allows for the detection of proteins through the identification of the fine structure of their amide I and II bands, up to the nanomolar concentration range. We exploited two distinct effects to enhance the sensitivity: (i) the concentration effect due to the presence of the superhydrophobic surface that conveys molecules dispersed in solution directly inside the focus of a FTIR spectromicroscope; (ii) the plasmonic resonance of the nanoantenna array that provides electromagnetic field enhancement in the amide I and II spectral region (1500–1700 cm−1). We demonstrate the detection of ferritin in the nanomolar concentration range, a blood protein that is usually available in small amounts in typical blood sample
An integrated superhydrophobic-plasmonic biosensor for mid-infrared protein detection at the femtomole level / Adele De, Ninno; Gabriele, Ciasca; Annamaria, Gerardino; Eugenio, Calandrini; Massimiliano, Papi; Marco De, Spirito; Nucara, Alessandro; Ortolani, Michele; Luca, Businaro; Baldassarre, Leonetta. - In: PHYSICAL CHEMISTRY CHEMICAL PHYSICS. - ISSN 1463-9076. - STAMPA. - (2015). [10.1039/C4CP05023A]
An integrated superhydrophobic-plasmonic biosensor for mid-infrared protein detection at the femtomole level
NUCARA, Alessandro;ORTOLANI, MICHELE;BALDASSARRE, Leonetta
2015
Abstract
In this work we present an integrated biosensor that enables FTIR (Fourier Transform-Infrared) detection of analytes contained in diluted solutions. The fabricated nanosensor allows for the detection of proteins through the identification of the fine structure of their amide I and II bands, up to the nanomolar concentration range. We exploited two distinct effects to enhance the sensitivity: (i) the concentration effect due to the presence of the superhydrophobic surface that conveys molecules dispersed in solution directly inside the focus of a FTIR spectromicroscope; (ii) the plasmonic resonance of the nanoantenna array that provides electromagnetic field enhancement in the amide I and II spectral region (1500–1700 cm−1). We demonstrate the detection of ferritin in the nanomolar concentration range, a blood protein that is usually available in small amounts in typical blood sampleFile | Dimensione | Formato | |
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