Brain surgery, and in particular epilepsy surgery, offers the unique opportunity to study viable human central nervous tissue in vitro. This does not only open a window to address the basic mechanisms underlying human disease, such as epilepsy, but it allows to venture into investigating neurophysiological functions per se. In the present paper, we describe the most commonly used methods in the electrophysiological (and, at least to some extent, also histochemical and molecular) analysis of human tissue in vitro. In addition, we consider the pitfalls and limitations of such studies, in particular regarding the issue of tissue sampling procedures and control experiments. (c) 2006 Elsevier B.V. All rights reserved.
Methodological approaches to exploring epileptic disorders in the human brain in vitro / Rüdiger, Kohling; Avoli, Massimo. - In: JOURNAL OF NEUROSCIENCE METHODS. - ISSN 0165-0270. - STAMPA. - 155:1(2006), pp. 1-19. [10.1016/j.jneumeth.2006.04.009]
Methodological approaches to exploring epileptic disorders in the human brain in vitro
AVOLI, Massimo
2006
Abstract
Brain surgery, and in particular epilepsy surgery, offers the unique opportunity to study viable human central nervous tissue in vitro. This does not only open a window to address the basic mechanisms underlying human disease, such as epilepsy, but it allows to venture into investigating neurophysiological functions per se. In the present paper, we describe the most commonly used methods in the electrophysiological (and, at least to some extent, also histochemical and molecular) analysis of human tissue in vitro. In addition, we consider the pitfalls and limitations of such studies, in particular regarding the issue of tissue sampling procedures and control experiments. (c) 2006 Elsevier B.V. All rights reserved.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
