Autophagy plays key roles during host defense against pathogens, but viruses have evolved strategies to block the process or to exploit it for replication and successful infection. The E5 oncoprotein of human papillomavirus type 16 (HPV16 E5) perturbs epithelial homeostasis down-regulating the expression of the keratinocyte growth factor receptor (KGFR/FGFR2b), whose signaling induces autophagy. Here we investigated the possible effects of 16E5 on autophagy in human keratinocytes expressing the viral protein. The 16E5 presence strongly inhibited the autophagic process, while forced expression and activation of KGFR counteracted this effect, demonstrating that the viral protein and the receptor exert opposite and interplaying roles not only on epithelial differentiation, but also in the control of autophagy. In W12 cells, silencing of the 16E5 gene in the context of the viral full length genome confirmed its role on autophagy inhibition. Finally, molecular approaches showed that the vir

HPV16 E5 deregulates the autophagic process in human keratinocytes / Belleudi, Francesca; Nanni, Monica; Raffa, Salvatore; Torrisi, Maria Rosaria. - In: ONCOTARGET. - ISSN 1949-2553. - STAMPA. - 6:11(2015), pp. 9370-9386. [10.18632/oncotarget.3326]

HPV16 E5 deregulates the autophagic process in human keratinocytes

BELLEUDI, Francesca;NANNI, MONICA;RAFFA, SALVATORE;TORRISI, Maria Rosaria
2015

Abstract

Autophagy plays key roles during host defense against pathogens, but viruses have evolved strategies to block the process or to exploit it for replication and successful infection. The E5 oncoprotein of human papillomavirus type 16 (HPV16 E5) perturbs epithelial homeostasis down-regulating the expression of the keratinocyte growth factor receptor (KGFR/FGFR2b), whose signaling induces autophagy. Here we investigated the possible effects of 16E5 on autophagy in human keratinocytes expressing the viral protein. The 16E5 presence strongly inhibited the autophagic process, while forced expression and activation of KGFR counteracted this effect, demonstrating that the viral protein and the receptor exert opposite and interplaying roles not only on epithelial differentiation, but also in the control of autophagy. In W12 cells, silencing of the 16E5 gene in the context of the viral full length genome confirmed its role on autophagy inhibition. Finally, molecular approaches showed that the vir
2015
FGFR2b; HPV; KGF/FGF7; KGFR; autophagy
01 Pubblicazione su rivista::01a Articolo in rivista
HPV16 E5 deregulates the autophagic process in human keratinocytes / Belleudi, Francesca; Nanni, Monica; Raffa, Salvatore; Torrisi, Maria Rosaria. - In: ONCOTARGET. - ISSN 1949-2553. - STAMPA. - 6:11(2015), pp. 9370-9386. [10.18632/oncotarget.3326]
File allegati a questo prodotto
File Dimensione Formato  
Belleudi_HPV16_2015.pdf

solo gestori archivio

Tipologia: Abstract
Licenza: Tutti i diritti riservati (All rights reserved)
Dimensione 10.24 MB
Formato Adobe PDF
10.24 MB Adobe PDF   Contatta l'autore

I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.

Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11573/778103
Citazioni
  • ???jsp.display-item.citation.pmc??? 25
  • Scopus 37
  • ???jsp.display-item.citation.isi??? 36
social impact