Leishmania infantum is a protozoan of the trypanosomatid family causing visceral leishmaniasis in the Mediterranean region. Leishmania parasites are transmitted by the bite of phlebotomine sand flies to the human host and are phagocyted by macrophages. The parasites synthesize N1-N8 bis(glutationyl) – spermidine (trypanothione) which furnishes electrons to the couple tryparedoxin-tryparedoxin peroxidase to reduce the reactive oxygen species produced by macrophages. Trypanothione is kept reduced by trypanothione reductase (TR) a FAD-containing enzyme which therefore is essential for parasite survival. In the TR reaction mechanism, NADPH binding and hydride transfer to FAD result in a charge-transfer cysteine disulphide intermediate, which thereafter reduces enzyme bound oxidized trypanothione.
|Titolo:||Leishmania infantum trypanothione reductase is a promiscuous enzyme carrying an nadph:o2 oxidoreductase activity shared by glutathione reductase|
MALATESTA, FRANCESCO (Corresponding)
|Data di pubblicazione:||2015|
|Appare nella tipologia:||01a Articolo in rivista|