Abstract BACKGROUND: The transcription factor DOF AFFECTING GERMINATION1 (DAG1) is a repressor of the light-mediated seed germination process. DAG1 acts downstream PHYTOCHROME INTERACTING FACTOR3-LIKE 5 (PIL5), the master repressor, and negatively regulates gibberellin biosynthesis by directly repressing the biosynthetic gene AtGA3ox1. The Dof protein DOF AFFECTING GERMINATION (DAG2) shares a high degree of aminoacidic identity with DAG1. While DAG1 inactivation considerably increases the germination capability of seeds, the dag2 mutant has seeds with a germination potential substantially lower than the wild-type, indicating that these factors may play opposite roles in seed germination. RESULTS: We show here that DAG2 expression is positively regulated by environmental factors triggering germination, whereas its expression is repressed by PIL5 and DAG1; by Chromatin Immuno Precipitation (ChIP) analysis we prove that DAG1 directly regulates DAG2. In addition, we show that Red light significantly reduces germination of dag2 mutant seeds. CONCLUSIONS: In agreement with the seed germination phenotype of the dag2 mutant previously published, the present data prove that DAG2 is a positive regulator of the light-mediated seed germination process, and particularly reveal that this protein plays its main role downstream of PIL5 and DAG1 in the phytochrome B (phyB)-mediated pathway.

DOF AFFECTING GERMINATION 2 is a positive regulator of light-mediated seed germination and is repressed by DOF AFFECTING GERMINATION 1 / Santopolo, Silvia; Boccaccini, Alessandra; Lorrai, Riccardo; Ruta, Veronica; Capauto, Davide; Minutello, Emanuele; Serino, Giovanna; Costantino, Paolo; Vittorioso, Paola. - In: BMC PLANT BIOLOGY. - ISSN 1471-2229. - STAMPA. - 15:(2015), pp. 1-11. [10.1186/s12870-015-0453-1]

DOF AFFECTING GERMINATION 2 is a positive regulator of light-mediated seed germination and is repressed by DOF AFFECTING GERMINATION 1

SANTOPOLO, SILVIA;BOCCACCINI, ALESSANDRA;LORRAI, RICCARDO;RUTA, VERONICA;CAPAUTO, DAVIDE;SERINO, Giovanna;COSTANTINO, Paolo;VITTORIOSO, Paola
2015

Abstract

Abstract BACKGROUND: The transcription factor DOF AFFECTING GERMINATION1 (DAG1) is a repressor of the light-mediated seed germination process. DAG1 acts downstream PHYTOCHROME INTERACTING FACTOR3-LIKE 5 (PIL5), the master repressor, and negatively regulates gibberellin biosynthesis by directly repressing the biosynthetic gene AtGA3ox1. The Dof protein DOF AFFECTING GERMINATION (DAG2) shares a high degree of aminoacidic identity with DAG1. While DAG1 inactivation considerably increases the germination capability of seeds, the dag2 mutant has seeds with a germination potential substantially lower than the wild-type, indicating that these factors may play opposite roles in seed germination. RESULTS: We show here that DAG2 expression is positively regulated by environmental factors triggering germination, whereas its expression is repressed by PIL5 and DAG1; by Chromatin Immuno Precipitation (ChIP) analysis we prove that DAG1 directly regulates DAG2. In addition, we show that Red light significantly reduces germination of dag2 mutant seeds. CONCLUSIONS: In agreement with the seed germination phenotype of the dag2 mutant previously published, the present data prove that DAG2 is a positive regulator of the light-mediated seed germination process, and particularly reveal that this protein plays its main role downstream of PIL5 and DAG1 in the phytochrome B (phyB)-mediated pathway.
arabidopsis-thaliana; transcription factor; phytochrome regulation; protein dag1; gene; gibberellin; expression; pil5; family; binding
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DOF AFFECTING GERMINATION 2 is a positive regulator of light-mediated seed germination and is repressed by DOF AFFECTING GERMINATION 1 / Santopolo, Silvia; Boccaccini, Alessandra; Lorrai, Riccardo; Ruta, Veronica; Capauto, Davide; Minutello, Emanuele; Serino, Giovanna; Costantino, Paolo; Vittorioso, Paola. - In: BMC PLANT BIOLOGY. - ISSN 1471-2229. - STAMPA. - 15:(2015), pp. 1-11. [10.1186/s12870-015-0453-1]
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11573/770992
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