The association of Epstein-Barr virus (EBV) with plasmacytoid malignancies is now well established but how the virus influences microRNA expression in such cells is not known. We have used multiple myeloma (MM) cell lines to address this issue and find that an oncomiR, miR-21 is induced after in vitro EBV infection. The PU.1 binding site in miR-21 promoter was essential for its activation by the virus. In accordance with its noted oncogenic functions, miR-21 induction in EBV infected MM cells caused downregulation of p21 and an increase in cyclin D3 expression. EBV infected MM cells were highly tumorigenic in SCID mice. Given the importance of miR-21 in plasmacytoid malignancies, our findings that EBV could further exacerbate the disease by inducing miR-21 has interesting implications both in terms of diagnosis and future miR based therapeutical approaches for the virus associated plasmacytoid tumors.

Epstein-Barr virus infection induces miR-21 in terminally differentiated malignant B cells / Anastasiadou, Eleni; Garg, Neha; Bigi, Rachele; Yadav, Shivangi; Campese, Antonio Francesco; Caterina, Lapenta; Massimo, Spada; Laura, Cuomo; Annalisa, Botta; Filippo, Belardelli; Frati, Luigi; Ferretti, Elisabetta; Faggioni, Alberto; Trivedi, Pankaj. - In: INTERNATIONAL JOURNAL OF CANCER. - ISSN 0020-7136. - ELETTRONICO. - (2015), pp. 1491-1497. [10.1002/ijc.29489]

Epstein-Barr virus infection induces miR-21 in terminally differentiated malignant B cells

ANASTASIADOU, ELENI;GARG, NEHA;BIGI, RACHELE;YADAV, SHIVANGI;CAMPESE, Antonio Francesco;FRATI, Luigi;FERRETTI, ELISABETTA;FAGGIONI, Alberto;TRIVEDI, PANKAJ
2015

Abstract

The association of Epstein-Barr virus (EBV) with plasmacytoid malignancies is now well established but how the virus influences microRNA expression in such cells is not known. We have used multiple myeloma (MM) cell lines to address this issue and find that an oncomiR, miR-21 is induced after in vitro EBV infection. The PU.1 binding site in miR-21 promoter was essential for its activation by the virus. In accordance with its noted oncogenic functions, miR-21 induction in EBV infected MM cells caused downregulation of p21 and an increase in cyclin D3 expression. EBV infected MM cells were highly tumorigenic in SCID mice. Given the importance of miR-21 in plasmacytoid malignancies, our findings that EBV could further exacerbate the disease by inducing miR-21 has interesting implications both in terms of diagnosis and future miR based therapeutical approaches for the virus associated plasmacytoid tumors.
2015
EBV; miR-21; multiple myeloma; plasma cells
01 Pubblicazione su rivista::01a Articolo in rivista
Epstein-Barr virus infection induces miR-21 in terminally differentiated malignant B cells / Anastasiadou, Eleni; Garg, Neha; Bigi, Rachele; Yadav, Shivangi; Campese, Antonio Francesco; Caterina, Lapenta; Massimo, Spada; Laura, Cuomo; Annalisa, Botta; Filippo, Belardelli; Frati, Luigi; Ferretti, Elisabetta; Faggioni, Alberto; Trivedi, Pankaj. - In: INTERNATIONAL JOURNAL OF CANCER. - ISSN 0020-7136. - ELETTRONICO. - (2015), pp. 1491-1497. [10.1002/ijc.29489]
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11573/768490
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