Our aim is to analyse changes in the level of neurotrophins (NGF and BDNF) in saliva samples of subjects performing the QMT training. NGF is essential for the development and phenotypic maintenance of neurons in the peripheral nervous system, and plays a pivotal role in relevant functions of cholinergic neurons of the basal forebrain: attention, arousal, motivation, memory and consciousness. ELISA assay and western blotting were utilised to measure NGF immunoreactivity and protein levels in the saliva samples of 25 subjects. We observed NGF decrease by ELISA analysis of saliva samples from QMT trainees (28 days) relative to controls. Western blotting analysis allowed us to determine that proNGF is the molecular form responsible for immunoreactivity, and to confirm the decrease observed. Our hypothesis is that the reduction of proNGF levels induced by QMT training is related to its maturation to NGF and utilisation, possibly favouring neuroplasticity versus neurodegeneration.
The molecular side of Quadrato / Venditti, Sabrina Donatella Sonia; L., Verdone; T., Dotan Ben Soussan; M., Caserta. - STAMPA. - (2013), pp. 12-12. (Intervento presentato al convegno First International Conference on Mindfulness tenutosi a Sapienza Università di Roma, Roma, ITALIA nel May 8-12 2013).
The molecular side of Quadrato
VENDITTI, Sabrina Donatella Sonia;
2013
Abstract
Our aim is to analyse changes in the level of neurotrophins (NGF and BDNF) in saliva samples of subjects performing the QMT training. NGF is essential for the development and phenotypic maintenance of neurons in the peripheral nervous system, and plays a pivotal role in relevant functions of cholinergic neurons of the basal forebrain: attention, arousal, motivation, memory and consciousness. ELISA assay and western blotting were utilised to measure NGF immunoreactivity and protein levels in the saliva samples of 25 subjects. We observed NGF decrease by ELISA analysis of saliva samples from QMT trainees (28 days) relative to controls. Western blotting analysis allowed us to determine that proNGF is the molecular form responsible for immunoreactivity, and to confirm the decrease observed. Our hypothesis is that the reduction of proNGF levels induced by QMT training is related to its maturation to NGF and utilisation, possibly favouring neuroplasticity versus neurodegeneration.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
