In this study, we have dissected the mature CD56 NK cell subsets based on the expression levels of CD16, one of the major NK cell activating receptor. We analyzed the phenotypic and functional profile of CD56brightCD16low, CD56brightCD16high, CD56dimCD16high and CD56dimCD16low NK cells in the peripheral blood (PB) and bone marrow (BM) from healthy donors who donated BM for transplantation. The results obtained indicated that CD56bright and CD56dimCD16low NK cells are more abundant in the BM with respect to the PB. Activating, inhibitory and cytokine receptors are expressed at lower levels on CD56dimCD16lowNK cells with respect to CD56brightCD16low/high. CD56dimCD16low NK cells express higher levels of CXCR4, whereas have no detectable expression of CX3CR1 as compared to CD56dimCD16high NK cells. Unlike CD56dimCD16high, CD56dimCD16low NK cells, either from BM or PB, can release IFN-γ following cytokine stimulation and are the major cytotoxic NK cell population upon binding to K562 targe
PHENOTYPIC AND FUNCTIONAL CHARACTERIZATION OF THE CD56DIMCD16LOW NK CELL SUBSET IN BONE MARROW AND PERIPHERAL BLOOD OF HEALTHY DONORS / Stabile, MARIA HELENA; Morrone, Stefania; Pagliara, D; Locatelli, F; Santoni, Angela; Gismondi, Angela. - ELETTRONICO. - (2013), pp. 243-243. (Intervento presentato al convegno International Congress of Immunology tenutosi a Milan nel August 22-27/2013).
PHENOTYPIC AND FUNCTIONAL CHARACTERIZATION OF THE CD56DIMCD16LOW NK CELL SUBSET IN BONE MARROW AND PERIPHERAL BLOOD OF HEALTHY DONORS.
STABILE, MARIA HELENA;MORRONE, Stefania;Locatelli F;SANTONI, Angela;GISMONDI, Angela
2013
Abstract
In this study, we have dissected the mature CD56 NK cell subsets based on the expression levels of CD16, one of the major NK cell activating receptor. We analyzed the phenotypic and functional profile of CD56brightCD16low, CD56brightCD16high, CD56dimCD16high and CD56dimCD16low NK cells in the peripheral blood (PB) and bone marrow (BM) from healthy donors who donated BM for transplantation. The results obtained indicated that CD56bright and CD56dimCD16low NK cells are more abundant in the BM with respect to the PB. Activating, inhibitory and cytokine receptors are expressed at lower levels on CD56dimCD16lowNK cells with respect to CD56brightCD16low/high. CD56dimCD16low NK cells express higher levels of CXCR4, whereas have no detectable expression of CX3CR1 as compared to CD56dimCD16high NK cells. Unlike CD56dimCD16high, CD56dimCD16low NK cells, either from BM or PB, can release IFN-γ following cytokine stimulation and are the major cytotoxic NK cell population upon binding to K562 targeI documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.