Introduction. In hematopoietic stem cells, growth and maturation of erythroid, granulocytic, monocytic and megakaryocytic lineages are largely controlled by unique combinations of transcription factors that cooperatively regulate promoters and enhancers present on specific target genes. miRNAs provide an additional level of control beyond the transcription factors and play a central role in hematopoietic differentiation through the establishment of complex regulatory circuitries. These small RNAs, to exert their function, are assembled in the functional RNA-induced silencing complexes (RISCs), where a member of Argonaute (Ago) family of proteins, Ago1-4, provides a unique platform for target recognition and gene silencing. Alteration of miRNAs levels and functional activity may affect proliferation, differentiation and genetic stability of hematopoietic stem/progenitor cells (HPCs), resulting in myeloproliferative disorders and leukaemia. Methods. By using myeloid cell lines and primar
ARGONAUTE-2 AS NOVEL MOLECULAR TARGET FOR THE DIFFERENTIATION THERAPY OF ACUTE MYELOID LEUKEMIA CELLS / Masciarelli, Silvia; Quaranta, R; Iosue', Ilaria; Colotti, G; Padula, Fabrizio; Varchi, G; Del Rio, A; Fazi, Francesco. - In: HAEMATOLOGICA. - ISSN 0390-6078. - STAMPA. - 99 S2:(2014), pp. 63-63. (Intervento presentato al convegno XIII Congress of the Italian Society of Experimental Hematology tenutosi a Rimini, Italy nel Rimini, Italy, October 15-17, 2014).
ARGONAUTE-2 AS NOVEL MOLECULAR TARGET FOR THE DIFFERENTIATION THERAPY OF ACUTE MYELOID LEUKEMIA CELLS
MASCIARELLI, SILVIA;IOSUE', ILARIA;PADULA, Fabrizio;FAZI, Francesco
2014
Abstract
Introduction. In hematopoietic stem cells, growth and maturation of erythroid, granulocytic, monocytic and megakaryocytic lineages are largely controlled by unique combinations of transcription factors that cooperatively regulate promoters and enhancers present on specific target genes. miRNAs provide an additional level of control beyond the transcription factors and play a central role in hematopoietic differentiation through the establishment of complex regulatory circuitries. These small RNAs, to exert their function, are assembled in the functional RNA-induced silencing complexes (RISCs), where a member of Argonaute (Ago) family of proteins, Ago1-4, provides a unique platform for target recognition and gene silencing. Alteration of miRNAs levels and functional activity may affect proliferation, differentiation and genetic stability of hematopoietic stem/progenitor cells (HPCs), resulting in myeloproliferative disorders and leukaemia. Methods. By using myeloid cell lines and primarI documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
