Abstract: Background: Acinetobacter baumannii has become an increasingly important nosocomial pathogen, particularly in intensive care units (ICUs). The aim of this investigation was to study the molecular epidemiology of A baumanii in a university hospital in Italy. Methods: All A baumanii isolates were collected and typed with phenotypic and genotypic methods during a 7-month period. A 1-year prospective surveillance of ICU-acquired infections was performed by using the National Nosocomial Infections Surveillance methodology. Results: A baumanni accounted for 28.4% of all infections and 46.7% of all pneumonia acquired in the ICU, with a nosocomial infection rate of 12.4% or 8 infections per 1000 patient-days. Risk factors for A baumannii acquisition in the ICU were mechanical ventilation and previous use of broad-spectrum antibiotics, whereas administration of carbapenems showed a significant protective effect. Pulsed-field gel electrophoresis of genomic ApaI digests identified at least 5 outbreaks in the ICU caused by 5 different clones, one replacing the other in a well-defined temporal order. Conclusions: Whereas the sequential temporal cluster of epidemic clones in the ICU is intriguing and requires further research, the clear evidence of cross-contamination of A baumannii isolates involved with infections in the ICU demands extensive preventive efforts.

Unusual genetic heterogeneity of Acinetobacter baumannii isolates in a university hospital in Italy / Villari, Paolo; Luigi, Iacuzio; Emilia Anna, Vozzella; Umberto, Bosco. - In: AMERICAN JOURNAL OF INFECTION CONTROL. - ISSN 0196-6553. - STAMPA. - 27:3(1999), pp. 247-253. [10.1053/ic.1999.v27.a96961]

Unusual genetic heterogeneity of Acinetobacter baumannii isolates in a university hospital in Italy

VILLARI, Paolo;
1999

Abstract

Abstract: Background: Acinetobacter baumannii has become an increasingly important nosocomial pathogen, particularly in intensive care units (ICUs). The aim of this investigation was to study the molecular epidemiology of A baumanii in a university hospital in Italy. Methods: All A baumanii isolates were collected and typed with phenotypic and genotypic methods during a 7-month period. A 1-year prospective surveillance of ICU-acquired infections was performed by using the National Nosocomial Infections Surveillance methodology. Results: A baumanni accounted for 28.4% of all infections and 46.7% of all pneumonia acquired in the ICU, with a nosocomial infection rate of 12.4% or 8 infections per 1000 patient-days. Risk factors for A baumannii acquisition in the ICU were mechanical ventilation and previous use of broad-spectrum antibiotics, whereas administration of carbapenems showed a significant protective effect. Pulsed-field gel electrophoresis of genomic ApaI digests identified at least 5 outbreaks in the ICU caused by 5 different clones, one replacing the other in a well-defined temporal order. Conclusions: Whereas the sequential temporal cluster of epidemic clones in the ICU is intriguing and requires further research, the clear evidence of cross-contamination of A baumannii isolates involved with infections in the ICU demands extensive preventive efforts.
1999
colonization; epidemiologic features; field gel-electrophoresis; intensive-care unit; macrorestriction analysis; nosocomial infections; outbreak; predominant pathogens; risk-factors; sao-paulo
01 Pubblicazione su rivista::01a Articolo in rivista
Unusual genetic heterogeneity of Acinetobacter baumannii isolates in a university hospital in Italy / Villari, Paolo; Luigi, Iacuzio; Emilia Anna, Vozzella; Umberto, Bosco. - In: AMERICAN JOURNAL OF INFECTION CONTROL. - ISSN 0196-6553. - STAMPA. - 27:3(1999), pp. 247-253. [10.1053/ic.1999.v27.a96961]
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