In insects, ABC transporters have been shown to contribute to defence/resistance to insecticides by reducing toxic concentrations in cells/tissues. Despite the extensive studies about this detoxifying mechanism, the temporal patterns of ABC transporter activation have been poorly investigated. Using the malaria vector Anopheles stephensi as a study system, we investigated the expression profile of ABC genes belonging to different subfamilies in permethrin-treated larvae at different time points (30 min to 48 h). Our results showed that the expression of ABCB and ABCG subfamily genes was upregulated at 1 h after treatment, with the highest expression observed at 6 h. Therefore, future investigations on the temporal dynamics of ABC gene expression will allow a better implementation of insecticide treatment regimens, including the use of specific inhibitors of ABC efflux pumps.
Temporal dynamics of the ABC transporter response to insecticide treatment: insights from the malaria vector Anopheles stephensi / S., Epis; Porretta, Daniele; Mastrantonio, Valentina; Urbanelli, Sandra; D., Sassera; L., De Marco; V., Mereghetti; M., Montagna; I., Ricci; G., Favia; C., Bandi. - In: SCIENTIFIC REPORTS. - ISSN 2045-2322. - ELETTRONICO. - 4:(2014), pp. 1-5. [10.1038/srep07435]
Temporal dynamics of the ABC transporter response to insecticide treatment: insights from the malaria vector Anopheles stephensi
PORRETTA, DANIELE;MASTRANTONIO, VALENTINA;URBANELLI, Sandra;
2014
Abstract
In insects, ABC transporters have been shown to contribute to defence/resistance to insecticides by reducing toxic concentrations in cells/tissues. Despite the extensive studies about this detoxifying mechanism, the temporal patterns of ABC transporter activation have been poorly investigated. Using the malaria vector Anopheles stephensi as a study system, we investigated the expression profile of ABC genes belonging to different subfamilies in permethrin-treated larvae at different time points (30 min to 48 h). Our results showed that the expression of ABCB and ABCG subfamily genes was upregulated at 1 h after treatment, with the highest expression observed at 6 h. Therefore, future investigations on the temporal dynamics of ABC gene expression will allow a better implementation of insecticide treatment regimens, including the use of specific inhibitors of ABC efflux pumps.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
