This work presents a GC–MS–MS–MS method for the direct determination of clenbuterol in human urine. The method 3 comprises a pretreatment procedure and the instrumental analysis of the derivatives performed by GC–MS (ion trap) with 3 electron impact ionization. The GC–MS analysis allows isolation and characterization of specific fragments from the 1 original (MS ) molecular structure, and in particular, those fragments originating from the precursor ion cluster (m/z5335– 2 337) characteristic of clenbuterol. The MS product fragment m/z5300 is in turn used as a further precursor fragment 3 4 giving rise to a MS spectrum specific for clenbuterol. MS fragmentation spectra were also investigated. However, further 3 4 fragmentation of MS product ions does not lead to functional MS spectra nor to any significant increase in the 3 signal-to-noise ratio. The sensitivity limit of the MS technique is lower than 0.2 mg/ l, with a linear range between 0.5 and 5 mg/ l, thus matching the basic requirements for antidoping analysis according to the guidelines of the International Olympic Committee. Due to its overall analytical performance, the method is presently being evaluated as a confirmation protocol to be followed to detect illicit clenbuterol administration to the athletes, and compared with reference GC–MS and GC–MS–MS techniques.
Rapid Determination of Diuretics in Human Urine by Gas Chromatography – Mass Spectrometry Following Microwave Assisted Derivatization / Amendola, L.; Colamonici, C.; Mazzarino, M.; Botre', Francesco. - In: ANALYTICA CHIMICA ACTA. - ISSN 0003-2670. - STAMPA. - 475:(2003), pp. 125-136. [10.1016/S0003-2670(02)01223-0]
Rapid Determination of Diuretics in Human Urine by Gas Chromatography – Mass Spectrometry Following Microwave Assisted Derivatization
BOTRE', Francesco
2003
Abstract
This work presents a GC–MS–MS–MS method for the direct determination of clenbuterol in human urine. The method 3 comprises a pretreatment procedure and the instrumental analysis of the derivatives performed by GC–MS (ion trap) with 3 electron impact ionization. The GC–MS analysis allows isolation and characterization of specific fragments from the 1 original (MS ) molecular structure, and in particular, those fragments originating from the precursor ion cluster (m/z5335– 2 337) characteristic of clenbuterol. The MS product fragment m/z5300 is in turn used as a further precursor fragment 3 4 giving rise to a MS spectrum specific for clenbuterol. MS fragmentation spectra were also investigated. However, further 3 4 fragmentation of MS product ions does not lead to functional MS spectra nor to any significant increase in the 3 signal-to-noise ratio. The sensitivity limit of the MS technique is lower than 0.2 mg/ l, with a linear range between 0.5 and 5 mg/ l, thus matching the basic requirements for antidoping analysis according to the guidelines of the International Olympic Committee. Due to its overall analytical performance, the method is presently being evaluated as a confirmation protocol to be followed to detect illicit clenbuterol administration to the athletes, and compared with reference GC–MS and GC–MS–MS techniques.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.