Celiac disease is a chronic disorder caused by dietary gluten in genetically susceptible individuals characterized by an inappropriate immune response. Recently, a new approach based on the detection of serum antibodies towards peptides of gluten (epitopes) has been reported. Here, we present a compact device based on an array of amorphous silicon (a-Si:H) photosensors where different epitopes, which establish immunoreaction in celiac patients, were immobilized on the surface of the device through the deposition of poly(2-hydroxyethyl methacrylate) (PHEMA)polymer brushes. The diagnosis of the celiac disease relies on the detection of the immunoreactions by using a chemiluminescent method. The results demonstrate the specificity and selectivity of the proposed system and show the successful integration of the recognition and detection elements in a single chip to obtain a portable low cost device as a novel tool for the diagnosis of celiac disease.
Array of Amorphous Silicon Photosensors for the Diagnosis of Celiac Disease in Lab-on-Chip System / Caputo, D., Scipinotti, R., A., D.P., Costantini, F., Manetti, C., Nascetti, A., Zahra, A., DE CESARE, G.. - ELETTRONICO. - (2013), pp. 77-78. (45th Conference "Gruppo Elettronica" Udine - Italia 17-21 giu 2013).
Array of Amorphous Silicon Photosensors for the Diagnosis of Celiac Disease in Lab-on-Chip System
CAPUTO, Domenico;SCIPINOTTI, RICCARDO;COSTANTINI, FRANCESCA;MANETTI, Cesare;NASCETTI, Augusto;ZAHRA, ANDLEEB;DE CESARE, Giampiero
2013
Abstract
Celiac disease is a chronic disorder caused by dietary gluten in genetically susceptible individuals characterized by an inappropriate immune response. Recently, a new approach based on the detection of serum antibodies towards peptides of gluten (epitopes) has been reported. Here, we present a compact device based on an array of amorphous silicon (a-Si:H) photosensors where different epitopes, which establish immunoreaction in celiac patients, were immobilized on the surface of the device through the deposition of poly(2-hydroxyethyl methacrylate) (PHEMA)polymer brushes. The diagnosis of the celiac disease relies on the detection of the immunoreactions by using a chemiluminescent method. The results demonstrate the specificity and selectivity of the proposed system and show the successful integration of the recognition and detection elements in a single chip to obtain a portable low cost device as a novel tool for the diagnosis of celiac disease.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
