To detect the interactions of DNA with the nuclear matrix proteins, DNA-protein cross-linkages were induced in intact nuclei from chicken liver by the use of cis-diammine dichloroplatinum. Methods have been devised for fast purification both of the proteins and of the DNA fragments involved in the cross-linked complexes. By Southern-Western blotting a number of matrix proteins isolated from the complexes have been shown to recognize specifically DNA sequences present in the cross-linked DNA fragments. This experimental approach not only allows to identify the nuclear matrix-DNA interactions existing in the nucleus before its disruption, but also provides a preparation of matrix proteins enriched in those species which are involved in such interactions and which can therefore be detected with high sensitivity.
DNA-nuclear matrix interactions analyzed by cross-linking reactions in intact nuclei from avian liver / Ferraro, Anna; Eufemi, Margherita; Cervoni, Laura; Altieri, Fabio; Turano, Carlo. - In: ACTA BIOCHIMICA POLONICA. - ISSN 0001-527X. - STAMPA. - 42(1995), pp. 145-152.
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Titolo: | DNA-nuclear matrix interactions analyzed by cross-linking reactions in intact nuclei from avian liver | |
Autori: | ||
Data di pubblicazione: | 1995 | |
Rivista: | ||
Citazione: | DNA-nuclear matrix interactions analyzed by cross-linking reactions in intact nuclei from avian liver / Ferraro, Anna; Eufemi, Margherita; Cervoni, Laura; Altieri, Fabio; Turano, Carlo. - In: ACTA BIOCHIMICA POLONICA. - ISSN 0001-527X. - STAMPA. - 42(1995), pp. 145-152. | |
Handle: | http://hdl.handle.net/11573/69841 | |
Appartiene alla tipologia: | 01a Articolo in rivista |