Contrast media (CM)-induced nephropathy (CIN) is an acute deterioration of renal function following administration of CM mediated to a large extent by the increased production of ROS within the kidney. Aim of this study was to evaluate whether a novel isoform of a recombinant Manganese SOD (rMnSOD) could provide an effective protection against CIN; this molecule shares the same ability of physiological SODs in scavenging reactive oxygen species (ROS) but, due to its peculiar properties, enters inside the cells after its administration. We studied the effects rMnSOD on oxidative damage in a rat model of CIN in uninephrectomized rats, that were randomly assigned to 3 experimental Groups: Group CON, control rats treated with the vehicle of CM, Group HCM, rats treated with CM and Group SOD, rats treated with CM and rMnSOD. In normal rats, pretreatment with rMnSOD, reduced renal superoxide anion production, induced by the activation of NAPDH oxidase, by 84 % (p < 0.001). In rats of Group HCM, ROS production was almost doubled compared to rat of Group CON (p < 0.01) but returned to normal values in rats of Group SOD, where a significant increase of SOD activity was detected (+16 % vs HCM, p < 0.05). Administration of CM determined a striking fall of GFR in rats of Group HCM (-70 %, p < 0.001 vs CON), greatly blunted in Group SOD (-28 % vs CON, p < 0.01); this was associated with a lower presence of both tubular necrosis and intratubular casts in SOD-treated rats (both p < 0.01 vs Group HCM). Our data indicate that rMnSOD is able to reduce renal oxidative stress, thus preventing the reduction of GFR and the renal histologic damage that follows CM administration.

Effect of a recombinant manganese superoxide dismutase on prevention of contrast-induced acute kidney injury / Antonio, Pisani; Massimo, Sabbatini; Eleonora, Riccio; Roberta, Rossano; Michele, Andreucci; Clemente, Capasso; Viviana De, Luca; Vincenzo, Carginale; Bizzarri, Mariano; Antonella, Borrelli; Antonella, Schiattarella; Michele, Santangelo; Aldo, Mancini. - In: CLINICAL AND EXPERIMENTAL NEPHROLOGY. - ISSN 1342-1751. - STAMPA. - 18:3(2014), pp. 424-431. [10.1007/s10157-013-0828-2]

Effect of a recombinant manganese superoxide dismutase on prevention of contrast-induced acute kidney injury

BIZZARRI, Mariano;
2014

Abstract

Contrast media (CM)-induced nephropathy (CIN) is an acute deterioration of renal function following administration of CM mediated to a large extent by the increased production of ROS within the kidney. Aim of this study was to evaluate whether a novel isoform of a recombinant Manganese SOD (rMnSOD) could provide an effective protection against CIN; this molecule shares the same ability of physiological SODs in scavenging reactive oxygen species (ROS) but, due to its peculiar properties, enters inside the cells after its administration. We studied the effects rMnSOD on oxidative damage in a rat model of CIN in uninephrectomized rats, that were randomly assigned to 3 experimental Groups: Group CON, control rats treated with the vehicle of CM, Group HCM, rats treated with CM and Group SOD, rats treated with CM and rMnSOD. In normal rats, pretreatment with rMnSOD, reduced renal superoxide anion production, induced by the activation of NAPDH oxidase, by 84 % (p < 0.001). In rats of Group HCM, ROS production was almost doubled compared to rat of Group CON (p < 0.01) but returned to normal values in rats of Group SOD, where a significant increase of SOD activity was detected (+16 % vs HCM, p < 0.05). Administration of CM determined a striking fall of GFR in rats of Group HCM (-70 %, p < 0.001 vs CON), greatly blunted in Group SOD (-28 % vs CON, p < 0.01); this was associated with a lower presence of both tubular necrosis and intratubular casts in SOD-treated rats (both p < 0.01 vs Group HCM). Our data indicate that rMnSOD is able to reduce renal oxidative stress, thus preventing the reduction of GFR and the renal histologic damage that follows CM administration.
2014
oxygen radicals; contrast-induced nephropathy superoxide dismutase oxygen radicals; contrast-induced nephropathy; superoxide dismutase
01 Pubblicazione su rivista::01a Articolo in rivista
Effect of a recombinant manganese superoxide dismutase on prevention of contrast-induced acute kidney injury / Antonio, Pisani; Massimo, Sabbatini; Eleonora, Riccio; Roberta, Rossano; Michele, Andreucci; Clemente, Capasso; Viviana De, Luca; Vincenzo, Carginale; Bizzarri, Mariano; Antonella, Borrelli; Antonella, Schiattarella; Michele, Santangelo; Aldo, Mancini. - In: CLINICAL AND EXPERIMENTAL NEPHROLOGY. - ISSN 1342-1751. - STAMPA. - 18:3(2014), pp. 424-431. [10.1007/s10157-013-0828-2]
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11573/697077
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