A rapid, sensitive and simple procedure has been developed for determination of argininosuccinate (ASA). The assay is based upon the enzymatic cleavage of ASA in the presence of excess of argininosuccinate lyase (ASAL), HPLC separation and UV-detection of fumarate formed. The calibration curve, obtained using standards, derives from a linear correlation between the reaction rate and ASA concentration. The range of HPLC detection corresponds with the level of normal excretion of argininosuccinate in urine from the patients with argininosuccinate lyase deficiency (ASLD
A new assay for argininosuccinate in urine samples by high performance liquid chromatography / Girelli, Anna Maria; Carunchio, Vincenzo Tito; H., Costanzi. - In: ANALYTICAL LETTERS. - ISSN 0003-2719. - STAMPA. - 29:(1996), pp. 1365-1375. [10.1080/00032719608001487]
A new assay for argininosuccinate in urine samples by high performance liquid chromatography
GIRELLI, Anna Maria;CARUNCHIO, Vincenzo Tito;
1996
Abstract
A rapid, sensitive and simple procedure has been developed for determination of argininosuccinate (ASA). The assay is based upon the enzymatic cleavage of ASA in the presence of excess of argininosuccinate lyase (ASAL), HPLC separation and UV-detection of fumarate formed. The calibration curve, obtained using standards, derives from a linear correlation between the reaction rate and ASA concentration. The range of HPLC detection corresponds with the level of normal excretion of argininosuccinate in urine from the patients with argininosuccinate lyase deficiency (ASLDI documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
