Maize MON810, grown and commercialised worldwide, is the only cultivated GM event in the EU. Maize MON810, variety DKC6575, and the corresponding near-isogenic line Tietar were studied in different growth conditions, to compare their behaviour in response to drought. Main photosynthetic parameters were significantly affected by water stress in both GM and non –GM varieties to a similar extents. Though DKC6575 (GM) had a greater sensitivity in the early phase of stress response as compared with Tietar (non GM), after six days of stress they behaved similarly, and both varieties recovered from stress damage. Profiling gene expression in water deficit regimes and in a generalised water stress condition showed an up-regulation of many stress- responsive genes, but a greater number of differentially expressed genes was observed in Tietar, with genes belonging to transcription factor families and genes encoding HSPs, LEAs and detoxification enzymes. Since induction of these genes have been indicated from the literature as typical of stress responses, their activation in Tietar rather than in DKC6575 may be reminiscent of a more efficient response to drought. DKC6575 was also analysed for the expression of the transgene CryIAb (encoding the delta-endotoxin insecticidal protein) in water deficit conditions. In all the experiments, the CryIAb transcript was not influenced by water stress, but was expressed at a constant level.. This suggests that though possessing a different pattern of sensitivity to stress, the GM variety maintains the same expression level for the transgene.

Comparison of drought stress response and gene expression between a GM maize variety and a near-isogenic non-GM variety / Gullì, Mariolina; Salvatori, Elisabetta; Fusaro, Lina; Pellacani, Claudia; Manes, Fausto; Marmiroli, Nelson. - In: PLOS ONE. - ISSN 1932-6203. - ELETTRONICO. - 10(2):e0117073(2015), pp. 1-21. [10.1371/journal.pone.0117073]

Comparison of drought stress response and gene expression between a GM maize variety and a near-isogenic non-GM variety

Elisabetta Salvatori;Lina Fusaro;Claudia Pellacani;Fausto Manes;
2015

Abstract

Maize MON810, grown and commercialised worldwide, is the only cultivated GM event in the EU. Maize MON810, variety DKC6575, and the corresponding near-isogenic line Tietar were studied in different growth conditions, to compare their behaviour in response to drought. Main photosynthetic parameters were significantly affected by water stress in both GM and non –GM varieties to a similar extents. Though DKC6575 (GM) had a greater sensitivity in the early phase of stress response as compared with Tietar (non GM), after six days of stress they behaved similarly, and both varieties recovered from stress damage. Profiling gene expression in water deficit regimes and in a generalised water stress condition showed an up-regulation of many stress- responsive genes, but a greater number of differentially expressed genes was observed in Tietar, with genes belonging to transcription factor families and genes encoding HSPs, LEAs and detoxification enzymes. Since induction of these genes have been indicated from the literature as typical of stress responses, their activation in Tietar rather than in DKC6575 may be reminiscent of a more efficient response to drought. DKC6575 was also analysed for the expression of the transgene CryIAb (encoding the delta-endotoxin insecticidal protein) in water deficit conditions. In all the experiments, the CryIAb transcript was not influenced by water stress, but was expressed at a constant level.. This suggests that though possessing a different pattern of sensitivity to stress, the GM variety maintains the same expression level for the transgene.
2015
maize; transgenic maize; drought stress; ecophysiology; global gene expression; CryIAb expression
01 Pubblicazione su rivista::01a Articolo in rivista
Comparison of drought stress response and gene expression between a GM maize variety and a near-isogenic non-GM variety / Gullì, Mariolina; Salvatori, Elisabetta; Fusaro, Lina; Pellacani, Claudia; Manes, Fausto; Marmiroli, Nelson. - In: PLOS ONE. - ISSN 1932-6203. - ELETTRONICO. - 10(2):e0117073(2015), pp. 1-21. [10.1371/journal.pone.0117073]
File allegati a questo prodotto
File Dimensione Formato  
Gulli_Comparison_2015.pdf

accesso aperto

Tipologia: Versione editoriale (versione pubblicata con il layout dell'editore)
Licenza: Creative commons
Dimensione 536.58 kB
Formato Adobe PDF
536.58 kB Adobe PDF

I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.

Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11573/696478
Citazioni
  • ???jsp.display-item.citation.pmc??? 4
  • Scopus 15
  • ???jsp.display-item.citation.isi??? 11
social impact