We investigated the mechanism whereby expression of a transgene encoding a locally acting isoform of insulin-like growth factor 1 (mIGF-1) enhances repair of skeletal muscle damage. increased recruitment of proliferating bone marrow cells to injured MLC/ mIgf-1 transgenic muscles was accompanied by elevated bone marrow stem cell production in response to distal trauma. Regenerating MLC/mIgf-1 transgenic muscles contained increased cell populations expressing stem cell markers, exhibited accelerated myogenic differentiation, expressed markers of regeneration and readily converted cocultured bone marrow to muscle. These data implicate mIGF-1 as a powerful enhancer of the regeneration response, mediating the recruitment of bone marrow cells to sites of tissue damage and augmenting local repair mechanisms.
Stem cell-mediated muscle regeneration is enhanced by local isoform of insulin-like growth factor 1 / Musaro, A; Giacinti, C; Borsellino, G; Dobrowolny, G; Pelosi, L; Cairns, L; Ottolenghi, S; Cossu, G; Bernardi, G; Battistini, L; M., Molinaro; Rosenthal, N.. - In: PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA. - ISSN 0027-8424. - 101:(2004), pp. 1206-1210. [10.1073/pnas.0303792101]
Stem cell-mediated muscle regeneration is enhanced by local isoform of insulin-like growth factor 1
MUSARO A;GIACINTI C;DOBROWOLNY G;PELOSI L;
2004
Abstract
We investigated the mechanism whereby expression of a transgene encoding a locally acting isoform of insulin-like growth factor 1 (mIGF-1) enhances repair of skeletal muscle damage. increased recruitment of proliferating bone marrow cells to injured MLC/ mIgf-1 transgenic muscles was accompanied by elevated bone marrow stem cell production in response to distal trauma. Regenerating MLC/mIgf-1 transgenic muscles contained increased cell populations expressing stem cell markers, exhibited accelerated myogenic differentiation, expressed markers of regeneration and readily converted cocultured bone marrow to muscle. These data implicate mIGF-1 as a powerful enhancer of the regeneration response, mediating the recruitment of bone marrow cells to sites of tissue damage and augmenting local repair mechanisms.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.